公开(公告)号：EP2798085A4

公开(公告)日：2015-07-01

申请号：EP12861230

申请日：2012-12-28

申请人： QUEST DIAGNOSTICS INVEST INC ,  SOMALOGIC INC

发明人： BOCK CHRIS ,  AYERS DEBORAH ,  NIKRAD MALTI P ,  GAWANDE BHARAT NATHU ,  BERTINO JENNIFER C ,  SUN WEIMIN ,  STROM CHARLES M ,  PARK NOH JIN

IPC分类号： A61K31/7072 ,  C12N15/115 ,  C12Q1/68

CPC分类号： G01N33/57492 ,  C07H21/04 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/317 ,  C12N2310/3513 ,  C12N2310/3517 ,  C12N2320/10 ,  G01N2333/71 ,  G01N2800/52

摘要： The present invention provides aptamers that specifically bind to the EGF receptor in a sample, and diagnostic and analytical methods using those aptamers. In some embodiments, the aptamers include a 3' cap. In some embodiments, the 3' cap is an inverted deoxythymidine. In some embodiments the aptamers include a spacer and at least one moiety selected from the group consisting of binding pair member and a detectable label, wherein the spacer is attached to the 5'-end of the aptamer and the moiety is attached the 5' end of the spacer. In some embodiments the spacer is hexaethylene glycol. In some embodiments, the binding pair member biotin. In some embodiments the detectable label is a fluorophore.

公开(公告)号：EP2798090A4

公开(公告)日：2015-08-05

申请号：EP12863794

申请日：2012-12-27

申请人： QUEST DIAGNOSTICS INVEST INC

发明人： STROM CHARLES M

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6851 ,  C12Q2600/158 ,  C12Q2527/137 ,  C12Q2565/629

摘要： The present invention provides methods for analyzing large nucleic acids including chromosomes and chromosomal fragments. In one aspect, the present invention provides a method of nucleic acid analysis comprising the steps of (a) obtaining a sample of nucleic acid comprising at least one chromosome or fragment greater than about 1000 base pairs in length and containing a target region; (b) creating an emulsion in which each drop of the emulsion contains an average of between about 0-2, 0-1.75, 0-1.5, 0-1.0, 0-0.75, 0-0.5, or fewer chromosomes or fragments of step (a), (c) performing emulsion PCR, (d) quantifying the number of emulsion droplets containing amplified nucleic acid from the target region; (e) calculating the ratio of droplets containing amplified nucleic acid from the target region to total droplets; and (f) comparing the ratio of step (e) to a reference ratio representing a known genotype.

公开(公告)号：EP2531618A4

公开(公告)日：2013-09-25

申请号：EP11740443

申请日：2011-02-04

申请人： QUEST DIAGNOSTICS INVEST INC ,  HANTASH FERAS ,  SUN WEIMIN ,  TSAO DAVID C ,  ROOT DANA MARIE ,  STROM CHARLES M

发明人： HANTASH FERAS ,  SUN WEIMIN ,  TSAO DAVID C ,  ROOT DANA MARIE ,  STROM CHARLES M

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6883 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6858 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G06F19/10 ,  C12Q2525/151 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2537/16

公开(公告)号：EP2082055A4

公开(公告)日：2010-02-17

申请号：EP07755301

申请日：2007-04-11

申请人： QUEST DIAGNOSTICS INVEST INC ,  U S GENOMICS

发明人： HUANG DONGHUI ,  STROM CHARLES M ,  POTTS STEVEN J ,  ROOKE JENNY ELLEN

IPC分类号： C12P19/34 ,  B01D57/02 ,  C12Q1/68

CPC分类号： C12Q1/6827 ,  C12Q1/6858 ,  C12Q2565/629 ,  C12Q2565/125 ,  C12Q2525/151