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公开(公告)号：EP2069519A2
公开(公告)日：2009-06-17
申请号：EP07800190.6
申请日：2007-09-21
摘要： The present invention relates to a method for producing 2-O-glyceryl-α-D-glucopyranoside (αGG; Figure 1) from a glucosyl donor and a glucosyl acceptor comprising the steps: - providing a sucrose phosphorylase (EC 2.4.1.7), incubating said sucrose phosphorylase with a mixture comprising a glucosyl donor and glycerol as glucosyl acceptor and isolating and/or purifying 2-O-glyceryl-α-D-glucopyranoside.
摘要翻译： 本发明涉及从葡糖基供体和葡糖基受体生产2-O-甘油基-α-D-吡喃葡萄糖苷（αGG;图1）的方法，包括以下步骤： - 提供蔗糖磷酸化酶（EC 2.4.1.7）， 将所述蔗糖磷酸化酶与包含葡糖基供体和甘油的混合物一起温育为葡糖基受体并分离和/或纯化2-O-甘油基-α-D-吡喃葡萄糖苷。
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2.发明授权
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公开(公告)号：EP2509450A1
公开(公告)日：2012-10-17
申请号：EP10787459.6
申请日：2010-12-07
CPC分类号： A23L33/21 , A23L33/10 , A23L33/135 , A23V2002/00 , A23V2200/326 , A23V2250/214
摘要： The present invention relates to the use of 2-o-glyceryl-α-D- glucopyranoside as prebiotic.
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公开(公告)号：EP2509450B1
公开(公告)日：2014-06-11
申请号：EP10787459.6
申请日：2010-12-07
CPC分类号： A23L33/21 , A23L33/10 , A23L33/135 , A23V2002/00 , A23V2200/326 , A23V2250/214
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公开(公告)号：EP4306646A1
公开(公告)日：2024-01-17
申请号：EP22184055.6
申请日：2022-07-11
发明人： NIDETZKY, Bernd , PFEIFFER, Martin
摘要： The invention refers to method and means for the production of C-nucleotides or C-nucleoside from the corresponding N-nucleoside. Specifically provided is a method for the production of a C-nucleotide or C-nucleoside from the corresponding N-nucleoside, said method comprising the steps of:
a) providing an N-nucleoside and a phosphate donor;
b) adding an enzyme having phosphorylase activity, whereupon the N-nucleoside is converted into the corresponding nucleobase and sugar-phosphate moiety;
c) adding an enzyme having isomerase activity, whereupon the sugar-phosphate moiety is subjected to an intramolecular phosphoryl transfer; and
d) adding an enzyme having C-glycosidase activity, whereupon the nucleobase and the rearranged sugar-phosphate moiety yield the corresponding C-nucleoside-monophosphate.
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