公开(公告)号：EP3090047A1

公开(公告)日：2016-11-09

申请号：EP14876534.0

申请日：2014-12-31

申请人： Dow AgroSciences LLC

发明人： KUMAR, Sandeep ,  GUPTA, Manju ,  WRIGHT, Terry R. ,  JAYNE, Susan M. ,  SMITH, Doug A. ,  ALABED, Diaa

IPC分类号： C12N15/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/04 ,  C12N5/10 ,  C12N15/82 ,  C12N15/87

CPC分类号： C12N15/8216

摘要： The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. Hi-II.

摘要翻译： 玉蜀黍c.v. B73泛素-1（Z.mays c.v.B73 Ubi-1）启动子在植物中驱动高水平的组成型转基因表达。 重复使用相同的Z. mays c.v. 多基因构建体中的B73 Ubi-1启动子也可能导致基因沉默，从而使得转基因产物的有效性降低。 提供了基因调控启动子元件，构建体以及使用来自不同基因型Z.mays c.v.的Ubi-1启动子的基因调控元件在植物细胞和/或植物组织中表达转基因的方法。 HI-II。

公开(公告)号：EP3090046A1

公开(公告)日：2016-11-09

申请号：EP14876153.9

申请日：2014-12-31

申请人： Dow AgroSciences LLC

发明人： KUMAR, Sandeep ,  GUPTA, Manju ,  WRIGHT, Terry R. ,  JAYNE, Susan M. ,  SMITH, Doug A. ,  ALABED, Diaa

IPC分类号： C12N15/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/04 ,  C12N5/10 ,  C12N15/82 ,  C12N15/87

CPC分类号： C12N15/8216

摘要： The
Zea mays c.v. B73 Ubiquitin-1 (
Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same
Z.
mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different
Z. mays genotype,
Z. mays c.v. LLN37.