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11.发明公开A PROCESS FOR PURIFYING VITAMIN K DEPENDENT PROTEINS SUCH AS COAGULATION FACTOR IX 有权一种用于净化维生素K依赖性蛋白质如凝血因子IX过程
公开(公告)号：EP2553095A1
公开(公告)日：2013-02-06
申请号：EP11713216.7
申请日：2011-03-30
申请人： Octapharma AG
发明人： GILLJAM, Gustav , WINGE, Stefan
IPC分类号： C12N9/64
CPC分类号： C07K1/22 , C07K14/745 , C12N9/6424
摘要： A process of manufacturing a prion-free Vitamin K dependent Protein in a purification sequence employing chromatography characterized in that - at least one chromatography step is performed using a multimodal resin - providing a fraction containing Vitamin K dependent Protein in an aqueous solution; - contacting the fraction containing the Vitamin K dependent Protein with a multimodal resin at a pH between 6-9; - optionally washing the multimodal resin having the Vitamin K dependent Protein adsorbed with an aqueous washing buffer to wash away contaminants and retain the Vitamin K dependent Protein, before the Vitamin K dependent Protein is eluted; - the Vitamin K dependent Protein is eluting from the multimodal resin at a pH between 6 to 9 in a buffer comprising arginine; and - optionally collecting Vitamin K dependent Protein containing fractions in purified or enriched form.
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12.发明公开A PROCESS FOR ISOLATION AND PURIFICATION OF A TARGET PROTEIN FREE OF PRION PROTEIN (PRPSC) 有权分离精制朊病毒蛋白免费靶蛋白的方法（PRP SC）
公开(公告)号：EP2190486A2
公开(公告)日：2010-06-02
申请号：EP08787446.7
申请日：2008-08-25
申请人： Octapharma AG
CPC分类号： C07K1/22 , B01D15/3828 , B01D15/426 , C07K14/47
摘要： A process for isolation and purification of a target protein by chromatography wherein the chromatography removes or depletes prions (PrPsc ), comprising the steps of - contacting a potentially PrPsc-contaminated sample comprising a target protein with a multimodal chromatographic material; - setting buffer conditions so that the target protein is bound to the multimodal chromatographic material and whereas PrPsc is not binding to the multimodal chromatographic material; - followed by elution of the target protein.
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