公开(公告)号：EP2678661A1

公开(公告)日：2014-01-01

申请号：EP12706704.9

申请日：2012-02-16

申请人： Commissariat à l'Énergie Atomique et aux Énergies Alternatives ,  Biomérieux

发明人： DUPOY, Mathieu ,  JOLY, Pierre ,  MALLARD, Frédéric ,  MARCOUX, Pierre ,  MATHEY, Raphaël ,  NOVELLI-ROUSSEAU, Armelle

IPC分类号： G01N21/03 ,  G01N21/64

CPC分类号： C12Q1/04 ,  G01N21/6486 ,  G01N21/65 ,  G01N2021/035

摘要： The invention relates to a method for observing a sample, including the steps of: a) providing a volume (G, V) of a substantially transparent medium having a first refractive index (n
int ) and comprising a first surface (S1) that is convex, rounded, or faceted, and a second substantially planar surface (S2), said first surface separating said volume from a surrounding medium (MA) having a second refractive index (n
ext ) that is lower than said first index, the sample to be observed being contained inside said volume or being deposited onto said first surface; b) observing said sample through said second substantially planar surface; and c) determining the presence of a substance or an object in the sample by detecting, during said step b), a ring of light that correlates with said volume.