公开(公告)号：EP2764103B1

公开(公告)日：2015-08-19

申请号：EP13824232.6

申请日：2013-12-12

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Zhang, Feng

IPC分类号： C12N15/63

CPC分类号： C12N15/907 ,  A61K38/43 ,  A61K38/46 ,  A61K38/47 ,  C12N9/14 ,  C12N9/22 ,  C12N9/52 ,  C12N9/96 ,  C12N15/00 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/111 ,  C12N15/63 ,  C12N15/85 ,  C12N15/902 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2800/90 ,  C12Q1/68

摘要： The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.

公开(公告)号：EP2998400A1

公开(公告)日：2016-03-23

申请号：EP15176051.9

申请日：2013-12-12

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Zhang, Feng

IPC分类号： C12N15/63

CPC分类号： C12N15/907 ,  A61K38/43 ,  A61K38/46 ,  A61K38/47 ,  C12N9/14 ,  C12N9/22 ,  C12N9/52 ,  C12N9/96 ,  C12N15/00 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/111 ,  C12N15/63 ,  C12N15/85 ,  C12N15/902 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2800/90 ,  C12Q1/68

摘要： The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.

摘要翻译： 本发明提供用于改变靶基因序列和相关基因产物的表达的系统，方法和组合物。 提供了载体和载体系统，其中一些编码CRISPR复合物的一个或多个组分，以及用于设计和使用这些载体的方法。 还提供了在真核细胞中引导CRISPR复合物形成的方法以及利用CRISPR-Cas系统的方法。

公开(公告)号：EP2764103A2

公开(公告)日：2014-08-13

申请号：EP13824232.6

申请日：2013-12-12

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  Zhang, Feng

发明人： Zhang, Feng

IPC分类号： C12N15/63

CPC分类号： C12N15/907 ,  A61K38/43 ,  A61K38/46 ,  A61K38/47 ,  C12N9/14 ,  C12N9/22 ,  C12N9/52 ,  C12N9/96 ,  C12N15/00 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/111 ,  C12N15/63 ,  C12N15/85 ,  C12N15/902 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2800/90 ,  C12Q1/68

摘要： The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.

摘要翻译： 本发明提供用于改变靶基因序列和相关基因产物的表达的系统，方法和组合物。 提供了载体和载体系统，其中一些编码CRISPR复合物的一个或多个组分，以及用于设计和使用这些载体的方法。 还提供了在真核细胞中引导CRISPR复合物形成的方法以及利用CRISPR-Cas系统的方法。