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    PRODUCING ISOTHIOCYANATES FROM CALLUS SUSPENSION CULTURES
    32.
    发明公开
    PRODUCING ISOTHIOCYANATES FROM CALLUS SUSPENSION CULTURES 审中-公开

    公开(公告)号：EP3707993A1

    公开(公告)日：2020-09-16

    申请号：EP19162136.6

    申请日：2019-03-12

    申请人： Perpetuum CropScience BVBA

    发明人： HOFENK, Jeroen ,  TROCH, James

    IPC分类号： A01H6/20 ,  A01H4/00 ,  C12N5/04

    摘要： The present invention provides a method of producing isothiocyanates, The method comprises forming, in a semisolid or solid callus induction medium, compact callus aggregates from cells obtained from explant material of a plant of a Brassicales plant species. The method comprises transferring cells from the callus aggregates, e.g. transferring the callus aggregates, to a suspension culture in a liquid medium in a shake flask, the liquid medium containing at least one elicitor and/or adsorbent. The method comprises transferring, after culturing in the shake flask, cells from the suspension culture to a further suspension culture in a bioreactor containing the at least one elicitor and/or adsorbent. The method comprises extracting and/or purifying of at least one isothiocyanate from cells obtained from the bioreactor.

    METHOD FOR INDUCING HAPLOIDY AND USE THEREOF IN PLANT BREEDING
    35.
    发明公开
    METHOD FOR INDUCING HAPLOIDY AND USE THEREOF IN PLANT BREEDING 审中-实审

    公开(公告)号：EP4389896A1

    公开(公告)日：2024-06-26

    申请号：EP23822989.2

    申请日：2023-06-06

    申请人： China National Rice Research Institute

    发明人： WANG, Kejian ,  WANG, Chun ,  WEI, Xin

    IPC分类号： C12N15/29 ,  C12N15/84 ,  A01H5/10 ,  A01H6/46 ,  A01H6/20

    摘要： The present invention relates to the field of biotechnology and plant breeding, and in particular relates to a method using plant cell proliferation regulator gene to induce haploid, and a use thereof in plant breeding. In the present invention, expression profiles of unfertilized egg cells of rice and rice embryos 5 days after fertilization are analyzed,and 2 genes are discovered to have relatively high expression,OsCPROl and OsCPRO2.The research shows that these two genes can be utilized to induce the production of haploid descendants.Specifically,a promoter specifically expressed in egg cells controls the gene to convert a plant,obtaining induced haploid plant material of a positive transgenic plant.Furthermore,the induced haploid plant material can be combined with a MiMe system to obtain apomixes material,and thereby generate clone seeds.