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    DETECTION OF MISFOLDED PROTEINS
    53.
    发明公开
    DETECTION OF MISFOLDED PROTEINS 审中-公开

    公开(公告)号：EP4292654A2

    公开(公告)日：2023-12-20

    申请号：EP23189057.5

    申请日：2015-09-11

    申请人： Board of Regents of the University of Texas System ,  Amprion, Inc. ,  Jara, Claudio Soto ,  Shahnawaz, Mohammad ,  Lebovitz, Russell M. ,  Vollrath, Benedikt K.

    发明人： JARA, Claudio, Soto ,  SHAHNAWAZ, Mohammad ,  LEBOVITZ, Russell M. ,  VOLLRATH, Benedikt, K.

    IPC分类号： A61P25/28

    摘要： Methods and kits are provided for amplifying and detecting misfolded proteins from samples, for example, from patients having Alzheimer s Disease, Parkinson s Disease, and the like. For example, a method for determining a presence of soluble, misfolded protein in a sample may include contacting the sample with a monomeric, folded protein to form an incubation mixture; conducting an incubation cycle two or more times effective to form an amplified portion of misfolded protein; incubating the incubation mixture effective to cause misfolding and/or aggregation of at least a portion of the monomeric, folded protein; physically disrupting the incubation mixture effective to break up at least a portion of any protein aggregate present; and determining the presence of the soluble, misfolded protein in the sample by detecting at least a portion of the soluble, misfolded protein. The monomeric, folded protein and the soluble, misfolded protein may exclude prion protein (PrP) and isoforms thereof.