公开(公告)号：EP1626991A2

公开(公告)日：2006-02-22

申请号：EP04752961.5

申请日：2004-05-21

申请人： Morphotek, Inc.

发明人： GRASSO, Luigi ,  NICOLAIDES, Nicholas, C. ,  SASS, Philip, M.

IPC分类号： C07K16/28 ,  C12N15/13 ,  C12N5/12 ,  A61P35/00

CPC分类号： C07K16/3069 ,  A61K39/00

摘要： Monoclonal antibodies that specifically bind to the tetrameric form of the alpha-folate receptor and not the monomeric form are provided. The antibodies are useful in the treatment of certain cancers, particularly cancers that have increased cell surface expression of the alphafolate receptor ('FR-a'), such as ovarian cancer. Hybridoma cells expressing the monoclonal antibodies, antibody derivatives, such as chimeric and humanized monoclonal antibodies, antibody fragments, mammalian cells expressing the monoclonal antibodies, derivatives and fragments, and methods of detecting and treating cancer using the antibodies, derivatives and fragments also are provided.

公开(公告)号：EP1572971A2

公开(公告)日：2005-09-14

申请号：EP03786774.4

申请日：2003-11-14

申请人： Morphotek Inc.

发明人： GRASSO, Luigi ,  LIANG, Shaohong ,  NICOLAIDES, Nicholas, E. ,  SASS, Philip, M.

IPC分类号： C12N1/00

CPC分类号： C12N15/01 ,  C07K16/00 ,  C07K16/1282 ,  C07K16/243 ,  C07K16/2863 ,  C07K16/30 ,  C07K2317/21 ,  C07K2317/73 ,  C12N15/1024

摘要： The invention provides methods for generating high titers of high-affinity antibodies from hybridoma cells produced by fusing myeloma cells with in vitro immunized donor cells. The hybridoma cells or mammalian expression cells with cloned antibody genes from the hybridomas producing the high-affinity antibodies may be mismatch repair defective due to defects of endogenous mismatch repair subunits of through expression of a dominant negative allele of a mismatch repair gene which allows the hybridoma cell to be hypermutable, may be rendered hypermutable by chemical means, or may be naturally mismatch repair deficient. High-affinity antibodies and high titer producer cells producing antibodies may be prepared by the methods of the invention.

公开(公告)号：EP1343879A1

公开(公告)日：2003-09-17

申请号：EP00977013.2

申请日：2000-11-07

申请人： Morphotek Inc.

发明人： GRASSO, Luigi ,  NICOLAIDES, Nicholas, C. ,  SASS, Philip, M.

IPC分类号： C12N15/00 ,  C12N15/85

CPC分类号： C12N9/00 ,  C07K14/47

摘要： Dominant-negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into mammalian cells new cell lines with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation or introduction of mutations by chemical mutagens. These methods are useful for generating novel and highly active antimicrobial molecules as well as superior antimicrobial agents from pre-existing chemicals. These methods are also useful for generating cell lines expressing novel antimicrobials that are useful for pharmaceutical manufacturing.