利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

140 条记录 (耗时 0.025 秒)

    METHOD FOR DETECTING ANALYTES
    53.
    发明公开
    METHOD FOR DETECTING ANALYTES 有权
    检测方法的分析物

    公开(公告)号：EP1082613A1

    公开(公告)日：2001-03-14

    申请号：EP99955324.1

    申请日：1999-05-24

    申请人： DADE BEHRING INC.

    发明人： MEHTA, Harshvardhan, B. ,  KURN, Nurith

    IPC分类号： G01N33/543

    CPC分类号： G01N33/54306 ,  Y10S436/819 ,  Y10S436/821 ,  Y10S436/827 ,  Y10S436/828

    摘要： The invention relates to methods of determining the presence or amount of an analyte in a sample suspected of containing the analyte, said method comprising the steps of: (a) bringing together in an acqueous medium to form a mixture: (i) the sample; (ii) at least one specific binder for the analyte; (iii) a first binding agent coupled to either (1) exogenous analyte or (2) the specific binder for the analyte; (iv) a support comprising a second binding agent; (b) adding an activator to the mixture, wherein the activator binds the first binding agent and the second binding agent of the support to immobilize the first binding agent; (c) determining the amount of the analyte in the sample by detecting the immobilized first binding agent, the presence or amount thereof being related to the presence or amount of the analyte in the sample.

    METHODS FOR DETERMINING AMOUNTS OF NUCLEIC ACIDS
    55.
    发明公开
    METHODS FOR DETERMINING AMOUNTS OF NUCLEIC ACIDS 审中-公开
    方法定量的NUCLEIC

    公开(公告)号：EP0988400A1

    公开(公告)日：2000-03-29

    申请号：EP99934310.6

    申请日：1999-02-16

    申请人： DADE BEHRING INC.

    发明人： PATEL, Rajesh ,  ULLMAN, Edwin, F.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6844 ,  C12Q2537/143 ,  C12Q2525/313

    摘要： The present invention relates to a method for detecting a single stranded target polynucleotide. A combination is provided, which comprises (i) a medium suspected of containing said single stranded target polynucleotide, (ii) a first oligonucleotide probe and (iii) a second oligonucleotide probe. The first probe has a sequence P1 that is capable of hybridizing with a sequence PP1 of the second probe. The single stranded target polynucleotide has a sequence S1 that is capable of hybridizing to PP1 and a sequence S2 that is capable of hybridizing to a sequence PP2 of the second probe. The first probe is incapable of hybridizing to PP2 or the single stranded target polynucleotide. S1 and S2 or PP1 and PP2 are noncontiguous and one or both of the first probe and the second probe comprise members of a signal producing system. The binding of the single stranded target polynucleotide, if present, to the second probe alters a signal generated by the signal producing system. The combination is subjected to conditions under which the single stranded target polynucleotide, if present, hybridizes to the second probe and displaces the first probe. The signal is then detected. The present method may be used in conjunction with a method for amplifying a target polynucleotide or a plurality of different nucleotides. Kits for carrying out the above methods are also disclosed. The method is particularly applicable to the amplification and detection of RNA.

    LUMINESCENT SPECIFIC BINDING ASSAY
    58.
    发明公开
    LUMINESCENT SPECIFIC BINDING ASSAY 失效
    LUMINESZIERDES特异性结合分析

    公开(公告)号：EP0956507A1

    公开(公告)日：1999-11-17

    申请号：EP98901712.0

    申请日：1998-01-06

    申请人： DADE BEHRING INC.

    发明人： PANKRATZ, Thomas, John ,  STOUT, Richard, Wayne

    IPC分类号： G01N33

    CPC分类号： G01N33/585 ,  G01N33/582

    摘要： A luminescent specific binding assay method for detecting an analyte in whole blood. The assay is a heterogeneous sandwich or competitive assay in which a first binding reagent is labeled with a luminescent photoprotein and an immobilized second binding reagent serves as a capture reagent. The luminescent photoprotein, upon activation, emits light which may be detected by a luminescence detector. The assay is rapid, sensitive and may be performed on small sample volumes.

    APPARATUS AND METHOD FOR ANALYSIS
    59.
    发明公开
    APPARATUS AND METHOD FOR ANALYSIS 失效
    方法和设备分析

    公开(公告)号：EP0832436A1

    公开(公告)日：1998-04-01

    申请号：EP97920214.0

    申请日：1997-04-08

    申请人： DADE BEHRING INC.

    发明人： PARRIS, Norman, Alfred

    IPC分类号： G01N35

    CPC分类号： G01N35/08 ,  Y10T436/117497 ,  Y10T436/118339

    摘要： An analyzer apparatus for analyzing components of a sample has a conduit and an electrochemical sensor in the conduit. The sample is passed through the conduit using a carrier liquid that is immiscible with the sample and non-wetting of the inner wall of the conduit to reduce instabilities in the apparatus due to undue fluid movement.