公开(公告)号：EP1443318A1

公开(公告)日：2004-08-04

申请号：EP03028285.9

申请日：2003-12-10

申请人： Agilent Technologies Inc. (a Delaware Corporation)

发明人： Flory, Curt A.

IPC分类号： G01N15/12

CPC分类号： G01N33/48721 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y30/00

摘要： The present invention provides an apparatus and method for controlling the movement of a biopolymer translocating a nanopore. The invention provides a first electrode, a second electrode adjacent to the first electrode, a third electrode adjacent to the second electrode and a fourth electrode adjacent to the third electrode. The first electrode is in electrical connection with the third electrode to define a first set of electrodes and the second electrode is in electrical connection with the fourth electrode to define a second set of electrodes. A DC voltage and radio frequency voltage is applied to the first set of electrodes while an opposite DC voltage and phase shifted radio frequency voltage is applied to the second set of electrodes to produce an electric field between the first set of electrodes and the second set of electrodes. The electric field is used to control the movement of a biopolymer translocating a nanopore. A method for controlling the movement of a biopolymer is also disclosed.

摘要翻译： 本发明提供的装置和方法，用于控制一种生物聚合物易位的纳米孔的移动。 本发明提供的第一电极，第二电极，其邻近于所述第一电极，第三电极，其邻近于所述第二电极和第四电极，其邻近于所述第三电极。 所述第一电极与所述第三电极电连接，以限定第一组电极和所述第二电极与所述第四电极，以限定的第二组电极的电连接。 的DC电压与射频电压被施加到所述第一组电极的，而在相反的直流电压和相移射频电压被施加到所述第二组电极以产生电场，第一组电极和第二组之间 电极。 电场被用来控制生物聚合物易位的纳米孔的移动。 一种用于控制生物聚合物gibt盘的运动游离缺失方法。

公开(公告)号：EP1153279B1

公开(公告)日：2004-01-07

申请号：EP00907121.8

申请日：2000-02-03

申请人： Coulter International Corp.

发明人： HUSHER, Frederick, K. ,  SCHNEIDER, Gerard ,  RAMIREZ, Lazaro

IPC分类号： G01N15/12

CPC分类号： G01N15/1227 ,  H03B5/1218 ,  H03B5/1228 ,  H03B5/124 ,  H03B5/1296

摘要： A junction field effect transistor (JFET) RF oscillator-detector circuit generates an RF signal for an apparatus for conducting electrical measurements of particles contained in a carrier fluid passing through an aperture in a cytometer flow cell. The JFET oscillator includes a plurality of parallel-coupled JFETs having respectively different VDS vs. IDS characteristics, that are biased to operate at square law detection regions of their respective VDS vs. IDS characteristics. One JFET operates in Class C mode, while the other operates in Class AB mode. An RF resonant circuit is electrically coupled to the JFETs and to the measurement cell, and is operative to establish the frequency of an RF field applied to the measurement cell. An RF load change detection circuit is coupled to the RF resonator circuit and is operative to detect an RF load change associated with a modification of the RF field as a result of a particle within the measurement cell aperture.

公开(公告)号：EP1080362B1

公开(公告)日：2003-07-09

申请号：EP99922332.4

申请日：1999-05-17

申请人： Microbial Systems Limited

发明人： WOOD, Michael Anthony

IPC分类号： G01N15/12 ,  G01N15/14

CPC分类号： G01N15/1218 ,  G01N2015/1037 ,  G01N2015/1281

摘要： A combined impedance and fluorescence particle detecting system (10) comprising a plate (60) separating two chambers (52, 58), the plate having a small orifice (63) enabling flow of liquid sample between the chambers, and means (132, 134) for determining variations in impedance at the orifice due to the flow therethrough of particles within the liquid sample; a light source for irradiating the particles within or close to the orifice and a detector (114) for receiving light emitted by the particles; and preferably means for clearing the orifice without removal of the plate from the system.

公开(公告)号：EP1299706A1

公开(公告)日：2003-04-09

申请号：EP01947344.6

申请日：2001-06-06

申请人： UNIVERSITEIT GENT

发明人： DE BISSCHOP, Frank ,  VANDEWEGE, Jan ,  DE METS, Marc

IPC分类号： G01N15/12

CPC分类号： G01N15/1227 ,  G01N2015/1006 ,  G01N2015/1062 ,  G01N2015/129

摘要： An AC-based detection method and device, whereby an LF signal generator applies LF power in parallel to an active cell with a gate and a device matched in impedance to the active cell. The LF carrier signal applied to the active cell is modulated with a measurement signal when a particle to be measured passes through the gate . The outputs from the cell and the matched impedance device are fed to an AC common mode rejection device (32), thus generating a difference signal. The difference signal from the common mode rejection device is demodulated. The demodulated signal is filtered to remove components of the AC carrier signal and its harmonics to retrieve a signal relating to the size of the object passing through the gate of the active cell. The output of the method and the device is similar to the results obtained with a DC detector but avoids the problem of electrolysis caused by the DC current in the active cell.

公开(公告)号：EP0870187B1

公开(公告)日：2002-09-11

申请号：EP96944117.9

申请日：1996-12-27

申请人： Shine, Thomas, Adam ,  Shine, Ian, Basil

发明人： Shine, Thomas, Adam ,  Shine, Ian, Basil

IPC分类号： G01N15/12

CPC分类号： G01N15/12 ,  G01N2015/0073 ,  G01N2015/105 ,  G01N2015/1062 ,  G01N2015/1087 ,  G01N2015/1093

公开(公告)号：EP1005641A4

公开(公告)日：2002-08-07

申请号：EP98920381

申请日：1998-05-12

申请人： CDC TECHNOLOGIES INC

发明人： CARVER EDWARD L JR ,  DECAVA DAVID C

IPC分类号： G01N15/12 ,  G01N33/487 ,  G01N27/00

CPC分类号： G01N15/1218

摘要： A method and apparatus are provided for differentiating cells in an automated particle analyzer (10), such as for use in hematological analysis, by measuring apparent cell size (or volume), membrane integrity and intracellular complexity ("voltaic fragility"). The cells are suspended in a selected reagent mixture. A predetermined dc voltage is applied across a sensing orifice (36) to thereby create a dc electric field within and around the orifice (36), and the reagent mixture is passed through the center of the orifice (36). The magnitude of the change in current flowing through dc electric field is measured therethrough, and based on the measured change, a signal is generated for each cell which is indicative of the apparent complexity of the cell. A visual display (54) is generated based on the signals facilitating differentiation of the cells.

公开(公告)号：EP1206692A1

公开(公告)日：2002-05-22

申请号：EP00949449.3

申请日：2000-08-03

申请人： Larsen, Ulrik Darling

发明人： Larsen, Ulrik Darling

IPC分类号： G01N15/12

CPC分类号： G01N15/1209

摘要： Particle characterisation apparatus working on the Coulter principle is provided with a count wafer formed by micro-machining of silicon so that the count wafer can economically be incorporated in a disposable housing (10) adapted to contain and retain for disposal liquid pumped through the count wafer during particle characterisation operations. The housing may incorporate electrodes (38) (4) in each of two chambers (22, 24) separated by a wall (20) containing an orifice (36) formed in the count wafer.

摘要翻译： 在库尔特原理下工作的粒子表征装置配备有通过硅的微加工形成的计数晶片，使得计数晶片可以经济地结合到一次性外壳（10）中，该一次性外壳适于容纳和保留用于处理通过计数晶片泵送的液体 在粒子表征操作中。 壳体可以在由包含在计数晶片中形成的孔口（36）的壁（20）分开的两个腔室（22,24）中的每一个中包含电极（38）（4）。

公开(公告)号：EP1032817B1

公开(公告)日：2002-02-20

申请号：EP98955666.7

申请日：1998-11-16

申请人： Melet, François

发明人： Melet, François

IPC分类号： G01N15/12

CPC分类号： G01N15/1209 ,  Y10T436/101666

摘要： The invention concerns an automatic hematologic counting and analysing device characterised in that it is arranged in three sets: a pumping set comprising an air pump controlled by a motor (M1) and three pumps for lysis, diluent and sampling respectively actuated by the motor (M2), a diluting set comprising three basins for waste material, white corpuscles and red corpuscles respectively and a measuring set including a measuring chamber.

公开(公告)号：EP1032817A1

公开(公告)日：2000-09-06

申请号：EP98955666.7

申请日：1998-11-16

申请人： Melet, François

发明人： Melet, François

IPC分类号： G01N15/12

CPC分类号： G01N15/1209 ,  Y10T436/101666

摘要： The invention concerns an automatic hematologic counting and analysing device characterised in that it is arranged in three sets: a pumping set comprising an air pump controlled by a motor (M1) and three pumps for lysis, diluent and sampling respectively actuated by the motor (M2), a diluting set comprising three basins for waste material, white corpuscles and red corpuscles respectively and a measuring set including a measuring chamber.

公开(公告)号：EP0652428B1

公开(公告)日：2000-02-02

申请号：EP94402479.3

申请日：1994-11-03

申请人： Sysmex Corporation

发明人： Kosaka, Tokihiro ,  Takarada, Kaoru

IPC分类号： G01N15/12 ,  G01N15/14

CPC分类号： G01N15/14 ,  G01N15/1012 ,  G01N15/12 ,  G01N2015/1037