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    CONCEPT FOR SWITCHING OF SAMPLING RATES AT AUDIO PROCESSING DEVICES
    77.
    发明公开
    CONCEPT FOR SWITCHING OF SAMPLING RATES AT AUDIO PROCESSING DEVICES 审中-公开

    公开(公告)号：EP4328908A2

    公开(公告)日：2024-02-28

    申请号：EP24151606.1

    申请日：2015-08-14

    申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

    发明人： DÖHLA, Stefan ,  FUCHS, Guillaume ,  GRILL, Bernhard ,  MULTRUS, Markus ,  PIETRZYK, Grzegorz ,  RAVELLI, Emmanuel ,  SCHNELL, Markus

    IPC分类号： G10L19/16

    摘要： Audio decoder device for decoding a bitstream, the audio decoder device comprising:
    a predictive decoder for producing a decoded audio frame from the bitstream, wherein the predictive decoder comprises a parameter decoder for producing one or more audio parameters for the decoded audio frame from the bitstream and wherein the predictive decoder comprises a synthesis filter device for producing the decoded audio frame by synthesizing the one or more audio parameters for the decoded audio frame;
    a memory device comprising one or more memories, wherein each of the memories is configured to store a memory state for the decoded audio frame, wherein the memory state for the decoded audio frame of the one or more memories is used by the synthesis filter device for synthesizing the one or more audio parameters for the decoded audio frame; and
    a memory state resampling device configured to determine the memory state for synthesizing the one or more audio parameters for the decoded audio frame, which has a sampling rate, for one or more of said memories by resampling a preceding memory state for synthesizing one or more audio parameters for a preceding decoded audio frame, which has a preceding sampling rate being different from the sampling rate of the decoded audio frame, for one or more of said memories and to store the memory state for synthesizing of the one or more audio parameters for the decoded audio frame for one or more of said memories into the respective memory.

    SYNTHESIS OF FUNCTIONAL CYTOCHROME P450 MONOOXYGENASES USING A EUKARYOTIC CELL-FREE SYSTEM COMPRISING MEMBRANE VESICLES
    78.
    发明公开
    SYNTHESIS OF FUNCTIONAL CYTOCHROME P450 MONOOXYGENASES USING A EUKARYOTIC CELL-FREE SYSTEM COMPRISING MEMBRANE VESICLES 审中-公开

    公开(公告)号：EP4328302A1

    公开(公告)日：2024-02-28

    申请号：EP22192384.0

    申请日：2022-08-26

    申请人： Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

    发明人： KNAUER, Jan, Felix ,  KUBICK, Stefan ,  ZEMELLA, Anne

    IPC分类号： C12N9/02 ,  C12P21/02

    摘要： The invention relates to a method for preparing functional membrane attached monooxygenases in cell-free systems which comprises at least the following steps:
    a) providing a reaction medium comprising at least the following components:
    a nucleic acid template coding for a Cytochrome P450 monooxygenase classified as EC 1.14.-.-, an RNApolymerase, a eukaryotic cell lysate containing ER-derived membrane vesicles which comprise NADPH-Cytochrom-P450-oxidoreductase (CPR), optionally heme or heme derivatives, in particular heme complexes selected from the group comprising or consisting of hematin and hemin;
    b) performing a cell-free reaction to synthesize the monooxygenase protein, wherein the cell-free translation reaction is performed at a temperature in the range from 18 to 33°C.
    The invention further relates to a reaction medium for preparing a functional Cytochrome P450 monooxygenase classified as EC 1.14.-.- in a cell-free system comprising at least the following components:
    a nucleic acid template coding for a Cytochrome P450 classified as EC 1.14.-.-, an RNA polymerase, a eukaryotic cell lysate containing membrane vesicles which comprise NADPH-Cytochrom-P450-oxidoreductase (CPR), heme or heme derivatives, in particular heme complexes selected from the group comprising or consisting of hematin and hemin.