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    Human low affinity Fc epsilon-receptor, the isolation and purification thereof
    75.
    发明公开
    Human low affinity Fc epsilon-receptor, the isolation and purification thereof 失效
    低亲和力，其分离和纯化的人Fc受体小量。

    公开(公告)号：EP0257114A1

    公开(公告)日：1988-03-02

    申请号：EP86111581.4

    申请日：1986-08-21

    申请人： Kishimoto, Tadamitsu, Prof.

    发明人： Kishimoto, Tadamitsu ,  Suemura, Masaki, Dr. ,  Kikutani, Hitoshi, Dr. ,  Barsumian, Edward L., Dr.

    IPC分类号： C12N15/00 ,  C07K15/06 ,  C12P21/00 ,  C12Q1/68 ,  A61K37/02

    CPC分类号： C12Q1/6876 ,  A61K38/00 ,  A61K39/00 ,  C07K14/7056 ,  C07K16/2851

    摘要： The present invention is concernd with

    a) isolation and purification of the soluble IgE binding factor (Fc ε R) secreted or shed by lymphoblastoid cells,
    b) partial sequencing the obtained Fc ε R-receptor, isolating the thus obtained fragments and determination of the sequen­ces of the thus obtained fragments,
    c) preparation of an oligonucleotide encoding to one of the said partial sequences,
    d) isolating and identifying expression vehicles containing the gene coding for Fc ε -receptor, comprising the steps of:

    synthesizing cDNA from a RNA matrix derived from lympho­blastoid cells producing Fc ε -receptor mRNA,

    incorporating said synthesized cDNA in expression vehicles to form an expression vehicle bank,

    hydribizing said incorporated cDNA to identify those expres­sion vehicles which contain a gene coding for Fc ε -receptor, with a labeled probe comprising an oligonucleotide common to the gene for Fc ε --receptor,

    replication and expression of the thus obtained gene, and sequencing the thus obtained gene,
    e) determination of the gene coding for the active human low-affinity Fc ε -receptor utilising isolated cDNA sequence obtained from the vehicles from operation d),
    f) expression of the Fc ε R cDNA in Cos 7 cells and
    g) preparing an expression vector containing the DNA sequen­ces coding for a soluble fragment of the Fc ε -receptor and expressing said soluble fragments in a microorganism or in mammalian cells.

    The water-soluble fragment of the Fc ε -receptor is suitable for treatment of allergic reactions.

    摘要翻译： 本发明是concernd用）由淋巴母细胞分泌的或脱落的隔离和可溶性IgE结合因子（FC小量的R纯化）中，b）部分测序所获得的Fc的ε-R-受体，分离的由此获得的片段和判定 由此获得的片段的序列，c）中制备的寡核苷酸编码的，以分离和鉴定表达载体包含对Fc受体的ε-编码基因，其包括以下步骤的所述部分序列中的一个，D）：从RNA基质合成cDNA 从淋巴母细胞生产的Fc受体的ε-mRNA的结合有所述合成的cDNA插入表达载体衍生以形成表达载体银行，hydribizing所述并入的cDNA，以确定哪一个包含对Fc受体的ε-的编码基因表达的那些车辆，与标记的探针，其包含 寡核苷酸共同的基因对Fc小量--receptor，复制和表达的如此Ò btained基因，并测序如此获得的基因，E）确定所述基因编码的活性的人低亲和力的Fc小量利用从车辆获得从手术ð分离的cDNA序列的受体）中，f）在Fc小量 - [R cDNA的表达 在COS 7个细胞和g）准备含有编码与Fc受体小量的可溶性片段的DNA序列的表达载体和表达在微生物或哺乳动物细胞中所述可溶性片段。 在Fc受体小量的水溶性片段是适用于治疗过敏性反应。

    Monoclonal antibodies specific to surface receptor for IgE and hybrid cell lines producing these antibodies and use thereof
    76.
    发明公开
    Monoclonal antibodies specific to surface receptor for IgE and hybrid cell lines producing these antibodies and use thereof 失效
    特异性针对表面IgE受体的单克隆抗体，以及它们的生产的杂交细胞系及其用途。

    公开(公告)号：EP0254767A1

    公开(公告)日：1988-02-03

    申请号：EP86111488.2

    申请日：1986-08-19

    申请人： Kishimoto, Tadamitsu, Prof.

    发明人： Kishimoto, Tadamitsu, Prof. dr. ,  Suemura, Masaki, Dr. ,  Kikutani, Hitoshi, Dr. ,  Barsumian, Edward L., Dr.

    IPC分类号： C12P21/00 ,  G01N33/577 ,  C12N5/00 ,  C12N15/00

    CPC分类号： C07K16/283

    摘要： An object of the present application is to provide new anti-Fc ε R-antibodies which enable B cells to be identified at a specific differentiation stage as well enabling the different degrees of atopy and Fc ε R-receptor expression to be defined as a result by the showing the Fc ε R is a B cell specific phenotype marker, the expression of which is strictly correlated with the differentiation stage of B cells, especially with the isotype switching in B cells. Therefore, Fc ε R positive B cells can be recognized by detec­tion with the new antibodies of the expressed Fc ε R, e.g. the expressed Fc ε R in supernatant having a molecular weight of about 25 Kd, and the use thereof in an ELISA.

    摘要翻译： 本申请的一个目的是提供新的抗Fc的ε-R-抗体，所述抗体使B细胞在特定分化阶段被识别，以及使得能够被定义为结果的不同程度的特应性和Fc的ε-R-受体表达的 由示出的Fc小量R是B细胞表型的特定标志物，这是严格地B细胞，阁楼中B细胞同种型转换的分化阶段相关的所有表达。 因此，小量的Fc R正极的B细胞可通过检测与过表达的Fc小量R的新抗体所识别，E.G. 表示在ELISA中其具有分子量约25kD的，并且使用在上清液中的Fc小量R上。