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1.发明公开
公开(公告)号：EP3228625A4
公开(公告)日：2017-12-13
申请号：EP15877635
申请日：2015-10-28
发明人： YANG ANQUAN , WANG JING , ZHANG LIHUA
IPC分类号： C07K1/14 , C07K1/12 , C07K14/435
CPC分类号： C07K1/145 , C07K1/12 , C07K14/43504
摘要： The present invention involves a pearl protein preparation method and a water-soluble pearl protein and an acid-soluble pearl protein obtained by adopting this method. A pearl protein preparation method comprises the following steps: (1) grinding pearls into pearl powder; (2) performing ultra-high pressure homogenization to the nanoscale pearl powder using deionized water bath, proceeding with centrifugation at low temperature to obtain a water-soluble pearl extract a and sediments, and rinsing the sediments with ethanol solution; (3) dissolving the sediments obtained above in weak acid at certain temperature, and stirring at a low speed for reaction; performing centrifugation at low temperature, and obtaining a supernatant being an acid-soluble pearl extract b; (4) performing desalination to water-soluble pearl extract a and acid-soluble pearl extract b respectively; (5) freeze-drying the water-soluble pearl extract a and acid-soluble pearl extract b after desalination, and obtaining a water-soluble pearl protein and an acid-soluble pearl protein respectively. This invention uses nanoscale pearl powder, which provides raw materials for pearl skin care products and health care products.
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公开(公告)号：EP3108873A1
公开(公告)日：2016-12-28
申请号：EP14885101.7
申请日：2014-12-04
发明人： YANG, Anquan , WANG, Jing , ZHANG, Lihua , LUO, Jianfen , XIE, Min
摘要： A method for separating and preparing a pearl extract from pearls comprise the following steps: 1) pulverizing pearls to a nanometer scale, then adding deionized water, and micro-emulsifying pearl powder by means of cooperation of a pressure and a temperature; 2) then performing electrodialysis on a pearl powder emulsion, placing the deionized water in a concentrated water pool, placing the pearl powder emulsion in a fresh water pool, and performing cyclic decalcification and separation to obtain a calcium salt-containing solution and a pearl protein solution; 3) using the obtained calcium salt-containing solution as soluble edible calcium or a soluble edible calcium raw material; 4) adding a phosphate buffered saline into the obtained pearl protein solution to form a homogeneous mixture; and 5) first performing pre-processing, then placing the liquid mixture into a reaction vessel to carry out an enzyme-catalyzed reaction, killing enzyme in a boiling water bath centrifugally after the reaction finishes, and removing a supernatant liquid, namely, a pearl complex whitening factor solution
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3.发明公开PREPARATION METHOD FOR CONCHIOLIN, AND WATER-SOLUBLE CONCHIOLIN AND ACID-SOLUBLE CONCHIOLIN PREPARED BY USING METHOD 审中-公开使用方法制备的海胆素，水溶性海胆素和酸溶性海胆素的制备方法
公开(公告)号：EP3228625A1
公开(公告)日：2017-10-11
申请号：EP15877635.1
申请日：2015-10-28
发明人： YANG, Anquan , WANG, Jing , ZHANG, Lihua
IPC分类号： C07K1/14 , C07K14/435
CPC分类号： C07K14/43504 , C07K1/12 , C07K1/145 , C07K1/36
摘要： The present invention involves a pearl protein preparation method and a water-soluble pearl protein and an acid-soluble pearl protein obtained by adopting this method. A pearl protein preparation method comprises the following steps: (1) grinding pearls into pearl powder; (2) performing ultra-high pressure homogenization to the nanoscale pearl powder using deionized water bath, proceeding with centrifugation at low temperature to obtain a water-soluble pearl extract a and sediments, and rinsing the sediments with ethanol solution; (3) dissolving the sediments obtained above in weak acid at certain temperature, and stirring at a low speed for reaction; performing centrifugation at low temperature, and obtaining a supernatant being an acid-soluble pearl extract b; (4) performing desalination to water-soluble pearl extract a and acid-soluble pearl extract b respectively; (5) freeze-drying the water-soluble pearl extract a and acid-soluble pearl extract b after desalination, and obtaining a water-soluble pearl protein and an acid-soluble pearl protein respectively. This invention uses nanoscale pearl powder, which provides raw materials for pearl skin care products and health care products.
摘要翻译： 本发明涉及一种珠蛋白制备方法，采用该方法得到的水溶性珍珠蛋白和酸溶性珍珠蛋白。 一种珍珠蛋白制备方法，包括以下步骤：（1）将珍珠研磨成珍珠粉; （2）用去离子水浴对纳米级珍珠粉进行超高压均质化处理，低温离心处理得到水溶性珍珠母提取物a和沉淀物，用乙醇溶液冲洗沉淀物; （3）将上述得到的沉淀物溶解于一定温度的弱酸中，低速搅拌反应; 在低温下离心分离，得到酸溶性珍珠提取物b的上清液; （4）分别对水溶性珍珠提取物a和酸溶性珍珠提取物b进行脱盐处理; （5）将脱水后的水溶性珍珠提取物a和酸溶性珍珠提取物b冷冻干燥，分别得到水溶性珠蛋白和酸溶性珠蛋白。 本发明采用纳米级珍珠粉，为珍珠护肤品和保健品提供原料。
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公开(公告)号：EP3108873A4
公开(公告)日：2016-12-28
申请号：EP14885101
申请日：2014-12-04
发明人： YANG ANQUAN , WANG JING , ZHANG LIHUA , LUO JIANFEN , XIE MIN
摘要： A method for separating and preparing a pearl extract from pearls comprise the following steps: 1) pulverizing pearls to a nanometer scale, then adding deionized water, and micro-emulsifying pearl powder by means of cooperation of a pressure and a temperature; 2) then performing electrodialysis on a pearl powder emulsion, placing the deionized water in a concentrated water pool, placing the pearl powder emulsion in a fresh water pool, and performing cyclic decalcification and separation to obtain a calcium salt-containing solution and a pearl protein solution; 3) using the obtained calcium salt-containing solution as soluble edible calcium or a soluble edible calcium raw material; 4) adding a phosphate buffered saline into the obtained pearl protein solution to form a homogeneous mixture; and 5) first performing pre-processing, then placing the liquid mixture into a reaction vessel to carry out an enzyme-catalyzed reaction, killing enzyme in a boiling water bath centrifugally after the reaction finishes, and removing a supernatant liquid, namely, a pearl complex whitening factor solution
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公开(公告)号：EP3228625B1
公开(公告)日：2019-02-20
申请号：EP15877635.1
申请日：2015-10-28
发明人： YANG, Anquan , WANG, Jing , ZHANG, Lihua
IPC分类号： C07K1/14 , C07K14/435 , C07K1/12
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公开(公告)号：EP3269356A4
公开(公告)日：2018-01-17
申请号：EP15885229
申请日：2015-10-28
发明人： HUO GANG , DENG XUEYANG , MA PING
摘要： A cosmetic composition for whitening skin and a preparation method thereof. The composition comprises the following components: pearl powder, pearl extract, glycyrrhiza glabra aqueous solution, hydrolyzed conchiolin protein and pea extract. The contents of the pearl powder and the pearl extract are not both zero, and the glycyrrhiza glabra aqueous solution contains 0.5 to 5.0% of the glycyrrhiza glabra extract.
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7.发明公开
公开(公告)号：EP3269356A1
公开(公告)日：2018-01-17
申请号：EP15885229.3
申请日：2015-10-28
发明人： HUO, Gang , DENG, Xueyang , MA, Ping
摘要： A cosmetic composition for whitening skin and a preparation method thereof. The composition comprises the following components: pearl powder, pearl extract, glycyrrhiza glabra aqueous solution, hydrolyzed conchiolin protein and pea extract. The contents of the pearl powder and the pearl extract are not both zero, and the glycyrrhiza glabra aqueous solution contains 0.5 to 5.0% of the glycyrrhiza glabra extract.
摘要翻译： 一种美白用化妆品组合物及其制备方法。 该组合物包含以下组分：珍珠粉，珍珠提取物，光果甘草水溶液，水解贝壳蛋白和豌豆提取物。 珍珠粉和珍珠提取物的含量均不为零，光甘草水溶液含有甘草提取物甘草提取物0.5〜5.0％。
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8.发明授权METHOD FOR SEPARATING AND PREPARING PEARL EXTRACT FROM PEARLS 有权VERFAHREN ZUR TRENNUNG UND HERSTELLUNG VON PERLENEXTRAKTEN AUS PERLEN
公开(公告)号：EP3108873B1
公开(公告)日：2017-06-21
申请号：EP14885101
申请日：2014-12-04
发明人： YANG ANQUAN , WANG JING , ZHANG LIHUA , LUO JIANFEN , XIE MIN
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