摘要:
A method of detecting the presence of bioactive molecules in a fluid sample, comprising contacting a solution of a microorganism selected from the group consisting of a monocellular algae and a cyanobacteria with the fluid sample so as to obtain a formulation having a microorganism concentration of 200 000-1 x 10 7 cells/mL of fluid sample; incubating the formulation for 10 to 120 minutes at a pH of 7 to 12 and a temperature between 18 and 35°C; and measuring the fluorescence emitted by the formulation, whereby a fluorescence emitted in the sample that is lower than that in a control sample is an indication that the sample contains a bioactive molecule.
摘要:
In a test piece for detecting a nitrite by virtue of the diazo coupling reaction, which test piece has supported on a substrate an effective amount of at least one oxidizing agent selected from the group consisting of M XO, M XO3, M XO4, M 3H2XO6, wherin M denotes an atom capable of being converted into a monovalent cation and X denotes a halogen atom, ethylenediamine tetraacetic acid-metals, cuprous chloride, copper sulfate, copper acetate, mercury acetate and bismuth acetate in corporation with a diazotizing agent and a coupling agent.
摘要:
A strain of Neisserie gonorrhoeae ATCC 31953 is described which has characteristically poor growth on chocolate agar at a temperature range of about 30°C to about 37°C In a CO 2 stmosphere suitable for growth of N. gonorrhoeae. This strain is resistant to nalidixic acid at the 5-10 mog/ml level and resistant to streptomycin at the 1000 meg/ml level or greater. The strain is suitable for a method for the laboratory diagnosis of gonorrhea, comprising the steps of (1) preparing a test strain of the microorganism Neisseria gonorrhoeae which is competent for transformation, (2) extracting N. gonorrhoeae DNA from a patient's apecimen material or colonies suspected of being N. gonor- rhoeae whether in pure or mixed culture by treatment with a base which lyses N. gonorrhoeae, (3) adusting the pH of the extract of step (2) to a pH that is not toxic to the test strain, (4) applying the pH adjusted extract to the test strain before or after putting it on or into a biological medium suitable for growth of N. gonorrhoese, (5) maintaining the treated test strain at optimum N. gonorrhoeae growth conditions which are Inhibitory for the test strain and (6) observing for detectable growth of the treated test strain. The observance of growth Indicates detection of N. gonorrhoeae DNA. Also described is a test kit for performing the method.
摘要:
Methods and devices for monitoring the viability of a biofilm comprising Pseudomonas Aeruginosa bacteria are provided by detecting pyocyanin. The invention relates to electrochemical methods and devices that offer a simple and inexpensive alternative for immediate identification of bacterial infection due to the presence of Pseudomonas aeruginosa. In some embodiments, an inexpensive, disposable electrochemical sensor can be used to rapidly screen for the presence of P. aeruginosa in clinical wound effluent samples.
摘要:
A bacteria analysis apparatus comprising: a specimen preparation section for preparing a first measurement specimen from a sample by using a first enzyme; a detecting section for detecting bacteria included in the first measurement specimen; and an information processing section for outputting information for supporting determination or kind of bacteria included in the sample on the basis of the detection result of the first measurement specimen. A method and a computer program product is also disclosed.