公开(公告)号：US06300543B1

公开(公告)日：2001-10-09

申请号：US09147489

申请日：1999-05-12

申请人： David D. Cass ,  Locksley E. McGann ,  Guichang Zhang ,  John D. Laurie ,  Jerome P. Ranch ,  William J. Gordon-Kamm

发明人： David D. Cass ,  Locksley E. McGann ,  Guichang Zhang ,  John D. Laurie ,  Jerome P. Ranch ,  William J. Gordon-Kamm

IPC分类号： C12N1582

CPC分类号： C12N15/8201

摘要： Methodology is provided for the production of uniformly transformed plants capable of transmitting a foreign gene to progeny by sexual reproduction. A foreign gene is introduced into the zygote in an isolated embryo sac and a transformed plant is recovered. Alternatively, a foreign gene is introduced into an egg cell in an isolated embryo sac, the egg cell is fertilized with an isolated sperm cell and a transformed plant is recovered. Sperm cells may be transformed with a foreign gene, an egg cell in an isolated embryo sac is fertilized with the transformed sperm cells, or nuclei isolated from the transformed sperm cells, and a transgenic plant is recovered. Another method for the production of transgenic plants is transformation of an embryo in an isolated embryo sac. The transgenic plant produced by any one of these methods is homogeneously transformed and capable of transmitting the foreign gene to progeny by sexual reproduction.

摘要翻译： 提供方法用于生产能够通过有性繁殖将外来基因传播给后代的均匀转化植物。 将外源基因导入分离的胚囊中的受精卵并回收转化的植物。 或者，外源基因被引入到分离的胚囊中的卵细胞中，卵细胞用分离的精子细胞受精并回收转化的植物。 精子细胞可以用外源基因转化，分离的胚囊中的卵细胞用转化的精子细胞受精，或从转化的精子细胞中分离出核，并回收转基因植物。 用于生产转基因植物的另一种方法是在分离的胚囊中转化胚胎。 通过这些方法中的任何一种产生的转基因植物被均匀转化，并且能够通过有性繁殖将外源基因转移到后代。