公开(公告)号：US07482144B2

公开(公告)日：2009-01-27

申请号：US10341220

申请日：2003-01-13

申请人： Heikki Ojamo ,  Merja Penttila ,  Heikki Heikkila ,  Jaana Uusitalo ,  Marja Ilmen ,  Marja-Leena Sarkki ,  Maija-Leena Vehkomaki

发明人： Heikki Ojamo ,  Merja Penttila ,  Heikki Heikkila ,  Jaana Uusitalo ,  Marja Ilmen ,  Marja-Leena Sarkki ,  Maija-Leena Vehkomaki

IPC分类号： C12P7/18 ,  C12N7/16 ,  C12N1/18

CPC分类号： C12P7/18

摘要： The invention relates to a method for the production of xylitol, the method comprising (a1) providing (i) a microorganism having xylanolytic activity, and (ii) a microorganism capable of converting a pentose sugar to xylitol; or (a2) providing a microorganism having xylanolytic activity and being capable of converting a pentose sugar to xylitol, (b) culturing the microorganism of step (a1) (i) or the microorganism of step (a2) in a medium comprising polymer or oligomer materials containing pentose sugars in conditions sufficient for enabling hydrolysis of said polymers or oligomers by the microorganism; (c) producing xylitol in the microorganism of step (a1) (ii) or in the microorganism of step (a2) by bioconversion of the hydrolysis products obtained in step (b), and (d) recovering said xylitol produced. The invention also relates to a microorganism, which has xylanolytic activity and has been genetically modified (i) to enhance its xylanolytic activity, and (ii) to reduce its xylitol metabolism.

摘要翻译： 本发明涉及一种生产木糖醇的方法，所述方法包括（a1）提供（i）具有木聚糖分解活性的微生物和（ii）能够将戊糖转化成木糖醇的微生物; 或（a2）提供具有木聚糖分解活性并能够将戊糖转化为木糖醇的微生物，（b）在包含聚合物或低聚物的培养基中培养步骤（a1）（i）的微生物或步骤（a2）的微生物） 在足以使微生物水解所述聚合物或低聚物的条件下含有戊糖的材料; （c）通过步骤（b）中获得的水解产物的生物转化和（d）回收所生产的木糖醇，在步骤（a1）（ⅱ）的微生物中或在步骤（a2）的微生物中生产木糖醇。 本发明还涉及一种具有木聚糖分解活性且经遗传修饰的微生物（i）增强其木聚糖分解活性，和（ii）降低其木糖醇代谢。