公开(公告)号：US20050266447A1

公开(公告)日：2005-12-01

申请号：US11100722

申请日：2005-04-07

申请人： Jonathan Duvick ,  Maria Fedorova

发明人： Jonathan Duvick ,  Maria Fedorova

IPC分类号： C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12Q1/68

CPC分类号： C12Q1/6897 ,  C12N15/1086

摘要： Methods and compositions for identifying a nucleotide sequence that encodes a polypeptide that directly or indirectly modulates the activity of a transcriptional regulatory region are provided. The methods permit the efficient screening of a large number of candidate nucleotide sequences for potential modulators of transcription. In particular, a population of polynucleotide constructs comprising a common transcriptional regulatory region operably linked to a candidate sequence is introduced into a population of host cells, and each candidate sequence is screened for modulation in its expression level, for example, using methods that employ hybridization to a complementary DNA microarray. The expression level of the candidate sequence correlates to the activity of the transcriptional regulatory region. Thus, alterations in the expression level of a candidate sequence indicate that the sequence encodes a polypeptide that modulates the activity of the transcriptional regulatory region. Compositions for practicing the invention are further provided.

摘要翻译： 提供了用于鉴定编码直接或间接调节转录调节区活性的多肽的核苷酸序列的方法和组合物。 该方法允许有效筛选潜在的转录调控因子的大量候选核苷酸序列。 特别地，将包含可操作地连接到候选序列的常见转录调节区的多核苷酸构建体的群体引入宿主细胞群体中，并且筛选每个候选序列以其表达水平进行调节，例如使用采用杂交的方法 到互补的DNA微阵列。 候选序列的表达水平与转录调控区的活性相关。 因此，候选序列的表达水平的改变表明序列编码调节转录调节区活性的多肽。 进一步提供实施本发明的组合物。