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    Quantitative assay for detection of newly synthesized rna in a cell-free system and indentification of rna synthesis inhibitors
    1.
    发明申请
    Quantitative assay for detection of newly synthesized rna in a cell-free system and indentification of rna synthesis inhibitors 失效
    用于检测无细胞系统中新合成的rna的定量测定和rna合成抑制剂的鉴定

    公开(公告)号：US20070141558A1

    公开(公告)日：2007-06-21

    申请号：US10593683

    申请日：2005-03-23

    申请人： Mingjun Huang ,  Yongnian Sun ,  Wengan Yang

    发明人： Mingjun Huang ,  Yongnian Sun ,  Wengan Yang

    IPC分类号： C12Q1/70 ,  C12Q1/68

    CPC分类号： C12Q1/707 ,  C12N2770/24011 ,  C12N2770/24211 ,  C12Q1/6816

    摘要： Methods for detecting populations of newly synthesized and newly initiated RNAs in a cell-free system are described. Suitable templates for the populations of newly synthesized and newly initiated RNAs are viral replicon RNAs, particularly HCV viral replicon RNA. The newly synthesized and newly initiated RNAs may be formed in the presence of a labeled nucleotide analog suitable for detection and/or quantitation of the RNAs. The assay may be employed to identify small molecule inhibitors of RNA synthesis of positive strand RNA viruses such as Hepatitis C Virus.

    摘要翻译： 描述了在无细胞系统中检测新合成和新引发的RNA的群体的方法。 用于新合成和新引发的RNA的群体的合适模板是病毒复制子RNA，特别是HCV病毒复制子RNA。 新合成的和新引发的RNA可以在适于检测和/或定量RNA的标记的核苷酸类似物的存在下形成。 该测定可以用于鉴定正链RNA病毒如丙型肝炎病毒的RNA合成的小分子抑制剂。