公开(公告)号：US20230374162A1

公开(公告)日：2023-11-23

申请号：US18248345

申请日：2021-10-05

Applicant: AMGEN INC.

Inventor： Danyang GONG ,  Fernando GARCES ,  Zhulun WANG

IPC: C07K16/46 ,  C12N15/63

CPC classification number: C07K16/468 ,  C12N15/63 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2317/55 ,  C07K2317/622

Abstract: The ability to generate a single antibody-based construct that can recognize multiple targets simultaneously, is paramount to advance many therapeutics candidates to clinic. Often, this implies extensive protein design with vary degrees of success. In the case of multispecific antibody constructs, there are multiple modalities from which to choose and often multiple antigen binders as well. Described here is the discovery of new methods to optimally pair antigen binders with the proper format, including the selection of common light chains.

公开(公告)号：US20240182600A1

公开(公告)日：2024-06-06

申请号：US18556248

申请日：2022-04-19

Applicant: AMGEN INC.

Inventor： Danyang GONG ,  Bram ESTES ,  Zhulun WANG ,  Fernando GARCES

IPC: C07K16/46

CPC classification number: C07K16/468 ,  C07K2317/31 ,  C07K2317/526

Abstract: The clinical potential of multispecific antibodies like bispecific and trispecific antibodies shows great promise for targeting complex diseases. However, the generation of those molecules presents great challenges particularly in regard to achieving acceptable expression levels free from mis-paired polypeptides. The presently claimed invention is directed to multispecific antigen binding proteins which improve upon existing charge pair technologies by redistributing the engineered charges within the CH3 regions of a heteromultimer.