公开(公告)号：US20150361478A1

公开(公告)日：2015-12-17

申请号：US14764142

申请日：2014-01-28

Applicant: BASF ENZYMES LLC

Inventor： Cristina POP ,  Arne I. SOLBAK, JR. ,  Adrienne HUSTON DAVENPORT

IPC: C12Q1/42

CPC classification number: C12Q1/42 ,  C07K1/145 ,  C12Q1/25 ,  G01N33/573 ,  G01N2333/916

Abstract: An in-feed assay and buffers for extracting an enzyme from a feed pellet treated at high temperature is provided. Another embodiment of the invention is measuring the enzyme activity of the enzyme additive extracted from the animal feed, measuring the quantity of enzyme extracted from the animal feed, or measuring both. In one embodiment the enzyme is an animal feed additive, such as a phytase.

Abstract translation: 提供了进料测定法和用于从高温处理的进料颗粒中提取酶的缓冲液。 本发明的另一个实施方案是测量从动物饲料中提取的酶添加剂的酶活性，测量从动物饲料中提取的酶的量，或测量两者。 在一个实施方案中，酶是动物饲料添加剂，例如植酸酶。

公开(公告)号：US20160083700A1

公开(公告)日：2016-03-24

申请号：US14771143

申请日：2014-03-10

Applicant: BASF ENZYMES LLC

Inventor： Xuqiu TAN ,  Arne I. SOLBAK, JR.

IPC: C12N9/16

CPC classification number: C12N9/16 ,  C12Y301/03008

Abstract: Polypeptides having phytase activity and polynucleotide sequences encoding the phytases are provided. The gene expresses the phytase at a level of at least 7 g/L to 40 g/L. The phytase have higher specific activity, retain activity at low pH, and retain activity at high temperature. The phytase can be used in a variety of compositions including food, feed, pharmaceuticals, and cleaning.

Abstract translation: 提供了具有植酸酶活性的多肽和编码植酸酶的多核苷酸序列。 该基因以至少7g / L至40g / L的水平表达肌醇六磷酸酶。 植酸酶具有较高的比活性，在低pH下保留活性，并保持高温下的活性。 植酸酶可用于各种组合物，包括食物，饲料，药物和清洁。