公开(公告)号：US10196617B2

公开(公告)日：2019-02-05

申请号：US14504849

申请日：2014-10-02

Applicant: BASF ENZYMES, LLC

Inventor： Arne Solbak ,  Brian Steer ,  Mark Dycaico ,  Katie A. Kline ,  Axel Trefzer ,  Thomas Todaro ,  Fatima El-Farrah ,  Alberto Alvarado ,  Gerhard Frey

IPC: C12N9/16 ,  C12P7/10 ,  A23L33/18 ,  A23K20/189

Abstract: This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.

公开(公告)号：US10047350B2

公开(公告)日：2018-08-14

申请号：US15135732

申请日：2016-04-22

Applicant: BASF ENZYMES LLC

Inventor： Walter Callen ,  Toby Richardson ,  Gerhard Frey ,  Jay M. Short ,  Eric J. Mathur ,  Kevin A. Gray ,  Janne S. Kerovuo ,  Malgorzata Slupska

IPC: C12N9/28 ,  C12P19/14 ,  C12N9/26 ,  A21D8/04 ,  C12P19/04

CPC classification number: C12N9/2417 ,  A21D8/042 ,  C12N9/2414 ,  C12P19/04 ,  C12P19/14 ,  C12Y302/01001 ,  Y02E50/16 ,  Y02E50/17

Abstract: The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at acidic, neutral and alkaline pH and increased temperature.

公开(公告)号：US10066222B2

公开(公告)日：2018-09-04

申请号：US15636039

申请日：2017-06-28

Applicant: BASF ENZYMES LLC

Inventor： Walter Callen ,  Toby Richardson ,  Gerhard Frey ,  Carl A. Miller ,  Martin Kazaoka ,  Eric J. Mathur ,  Jay M. Short

IPC: C12N9/26 ,  C12P19/14 ,  C12N9/28

CPC classification number: C12N9/2411 ,  C12N9/2417 ,  C12P19/14

Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars.

公开(公告)号：US09701950B2

公开(公告)日：2017-07-11

申请号：US14719547

申请日：2015-05-22

Applicant: BASF ENZYMES LLC

Inventor： Walter Callen ,  Toby Richardson ,  Gerhard Frey ,  Carl A. Miller ,  Martin Kazaoka ,  Eric J. Mathur ,  Jay M. Short

IPC: C12N9/26 ,  C12N9/28 ,  C12P19/14

CPC classification number: C12N9/2411 ,  C12N9/2417 ,  C12P19/14

Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars.