公开(公告)号：US20220251628A1

公开(公告)日：2022-08-11

申请号：US17679310

申请日：2022-02-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. SAMUELS ,  Jeffrey Charles OLSON ,  Andrew WATSON ,  Keith BROWN ,  Darren R. LINK

IPC: C12Q1/6806 ,  C40B50/08 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804 ,  C12N15/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.