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    Leucine aminopeptidases produced recombinantly from Aspergillus soyae
    1.
    发明授权
    Leucine aminopeptidases produced recombinantly from Aspergillus soyae 失效
    亮氨酸氨基肽酶由大豆曲霉重组产生

    公开(公告)号：US06228632B1

    公开(公告)日：2001-05-08

    申请号：US09011540

    申请日：1998-04-20

    申请人： Erwin Schuster ,  Bruno Sproessler ,  Kornelia Titze ,  Michael Gottschalk ,  Nguyen Quoc Khanh ,  Sabine Wolf ,  Hermann Plainer

    发明人： Erwin Schuster ,  Bruno Sproessler ,  Kornelia Titze ,  Michael Gottschalk ,  Nguyen Quoc Khanh ,  Sabine Wolf ,  Hermann Plainer

    IPC分类号： C12N948

    CPC分类号： C12N9/48

    摘要： This invention relates to a recombinant deoxyribonucleic acid (DNA) which can be isolated from Aspergillus soyae, characterised in that it codes for a leucine aminopeptidase (LAP) and comprises a nucleotide sequence corresponding to the nucleotide sequence given in SEQ ID NO: 1 for the mature LAP or to a nucleotide sequence derived therefrom which hybridises under stringent conditions with the nucleotide sequence given in SEQ ID NO: 1 for the mature LAP. The invention further relates to vectors and transformed host organisms, and also relates to methods of producing LAP. Enzyme products for the production of protein hydrolysates, as well as protein hydrolysates which are produced correspondingly, also form part of the invention.

    摘要翻译： 本发明涉及可从大豆粉中分离的重组脱氧核糖核酸（DNA），其特征在于其编码亮氨酸氨基肽酶（LAP），其包含对应于SEQ ID NO：1中给出的核苷酸序列的核苷酸序列，用于 成熟LAP或衍生自其的核苷酸序列，其在严格条件下与成熟LAP的SEQ ID NO：1中给出的核苷酸序列杂交。 本发明进一步涉及载体和转化的宿主生物，并且还涉及产生LAP的方法。 用于生产蛋白质水解产物的酶产物以及相应生产的蛋白质水解产物也构成本发明的一部分。

    Protein with phospholipase activity
    2.
    发明授权
    Protein with phospholipase activity 有权
    具有磷脂酶活性的蛋白质

    公开(公告)号：US6140094A

    公开(公告)日：2000-10-31

    申请号：US142469

    申请日：1998-09-08

    申请人： Fridolin Loffler ,  Gerald Jungschaffer ,  Quoc Nguyen Khanh ,  Erwin Schuster ,  Bruno Sprossler ,  Sabine Wolf

    发明人： Fridolin Loffler ,  Gerald Jungschaffer ,  Quoc Nguyen Khanh ,  Erwin Schuster ,  Bruno Sprossler ,  Sabine Wolf

    IPC分类号： A21D2/26 ,  A21D8/04 ,  C11B3/00 ,  C12N1/15 ,  C12N9/18 ,  C12S3/18 ,  C12N9/20 ,  C07H21/04 ,  C07K1/00 ,  C12N1/20 ,  C12N15/00

    CPC分类号： A21D8/042 ,  A21D2/267 ,  C11B3/003 ,  C12N9/18 ,  Y10S435/913 ,  Y10S435/917

    摘要： This invention relates to a protein having phospholipase activity, which is characterised in that it has the mature sequence of Aspergillus lysophospholipase or a sequence derived therefrom and that it may be cleaved at at least one site, wherein, in the event of cleavage, the restriction fragments are optionally either linked by means of at least one bond cleavable under reducing conditions or at least one of the unlinked restriction fragments has phospholipase activity, and to a process for the production of this protein by fermenting a suitably transformed lysophospholipase-producing host organism in a suitable culture medium and isolating the protein having phospholipase activity from the cell-free culture filtrate, wherein fermentation is performed in the acidic to slightly alkaline range.

    摘要翻译： PCT No.PCT / EP98 / 00081 Sec。 371日期：1998年9月8日 102（e）1998年9月8日PCT PCT 1998年1月8日PCT公布。 公开号WO98 / 31790 日期1998年7月23日本发明涉及具有磷脂酶活性的蛋白质，其特征在于其具有成熟的曲霉溶血磷脂酶序列或其衍生的序列，并且其可在至少一个位点断裂，其中，在事件 的切割，限制性片段任选地通过至少一个在还原条件下可切割的键连接或至少一个未连接的限制性片段具有磷脂酶活性，以及​​通过发酵适当转化的溶血磷脂酶来生产该蛋白质的方法 - 在合适的培养基中产生宿主生物体，并从无细胞培养滤液中分离具有磷脂酶活性的蛋白质，其中发酵在酸性至略微碱性范围内进行。

    Method for producing a friction material mass and friction linings made therefrom
    5.
    发明授权
    Method for producing a friction material mass and friction linings made therefrom 失效
    用于制造摩擦材料块和由其制成的摩擦衬里的方法

    公开(公告)号：US07754001B2

    公开(公告)日：2010-07-13

    申请号：US11642102

    申请日：2006-12-20

    申请人： Christian Spandern ,  Sabine Wolf

    发明人： Christian Spandern ,  Sabine Wolf

    IPC分类号： C08J5/14 ,  C09K3/14 ,  C09K3/18 ,  C04B12/04 ,  C04B28/26 ,  C04B35/16

    CPC分类号： F16D69/02

    摘要： The invention concerns a method for producing a friction material mass, in particular for friction linings in vehicles, in which essentially fibrous material, fillers, lubricants, metallic components and binding agents are wet-processed. In order to be able to produce from the friction material mass friction linings with increased thermostability, water glass is used as binding agent.

    摘要翻译： 本发明涉及一种用于生产摩擦材料块的方法，特别是用于车辆中的摩擦衬里，其中基本上纤维材料，填料，润滑剂，金属组分和粘合剂被湿法处理。 为了能够从具有增加的热稳定性的摩擦材料质量摩擦衬里生产，使用水玻璃作为粘合剂。

    Method for producing a friction material mass and friction linings made therefrom
    6.
    发明申请
    Method for producing a friction material mass and friction linings made therefrom 失效
    用于制造摩擦材料块和由其制成的摩擦衬里的方法

    公开(公告)号：US20070105978A1

    公开(公告)日：2007-05-10

    申请号：US11642102

    申请日：2006-12-20

    申请人： Christian Spandern ,  Sabine Wolf

    发明人： Christian Spandern ,  Sabine Wolf

    IPC分类号： C08J5/14

    CPC分类号： F16D69/02

    摘要： The invention concerns a method for producing a friction material mass, in particular for friction linings in vehicles, in which essentially fibrous material, fillers, lubricants, metallic components and binding agents are wet-processed. In order to be able to produce from the friction material mass friction linings with increased thermostability, water glass is used as binding agent.

    摘要翻译： 本发明涉及一种用于生产摩擦材料块的方法，特别是用于车辆中的摩擦衬里，其中基本上纤维材料，填料，润滑剂，金属组分和粘合剂被湿法处理。 为了能够从具有增加的热稳定性的摩擦材料质量摩擦衬里生产，使用水玻璃作为粘合剂。

    Method for identifying bhs-specific proteins and fragments thereof
    7.
    发明申请
    Method for identifying bhs-specific proteins and fragments thereof 审中-公开
    用于鉴定bhs特异性蛋白质及其片段的方法

    公开(公告)号：US20060172300A1

    公开(公告)日：2006-08-03

    申请号：US10527788

    申请日：2003-03-08

    申请人： Sabine Wolf ,  Martina Jager ,  Thorsten Bangsow ,  Carmen Bangsow ,  Dominik Jordan ,  Bernhard Pelzer ,  Thomas Oppolzer

    发明人： Sabine Wolf ,  Martina Jager ,  Thorsten Bangsow ,  Carmen Bangsow ,  Dominik Jordan ,  Bernhard Pelzer ,  Thomas Oppolzer

    IPC分类号： C12Q1/68 ,  G01N33/00 ,  C07K14/705

    CPC分类号： C07K14/47 ,  A61K38/00

    摘要： The invention relates to a method for identifying the presence of BBB-specific protein/fragment in endothelial cells of brain capillaries, characterized in that a) endothelial cells of brain capillaries freshly isolated from brain are conventionally pre-purified by enzymatic digestion, b) the digest obtained in step a) is treated with a lysis buffer that essentially destroys present erythrocytes and apoptotic cells and maintains at least 70% of the endothelial cells of brain capillaries in vital form, c) the product obtained in step b) is optionally purified further, d) a subtractive cDNA library is prepared from the endothelial cells of brain capillaries and a subtractive tissue, e) a cDNA subtraction is performed using one ore more differential hybridization(s), f) clones from the subtractive cDNA library are verified by differential hybridization with respect to their respective expression, g) a complete cDNA sequence is prepared for the BBB-specific clones from the subtractive cDNA library, and h) the expression pattern of the investigated clones is compared between fresh and cultured endothelial cells of brain capillaries and, that way, the presence of BBB-specific proteins or fragments thereof is identified as well as proteins and fragments thereof identified with this method.

    摘要翻译： 本发明涉及用于鉴定脑毛细血管内皮细胞中BBB特异性蛋白/片段的存在的方法，其特征在于a）通过酶消化常规地预先纯化来自脑中新鲜分离的脑毛细血管的内皮细胞，b） 在步骤a）中获得的消化物质用基本上破坏现有红细胞和凋亡细胞的裂解缓冲液处理，并将至少70％的脑毛细血管内皮细胞维持为重要形式，c）步骤b）中获得的产物任选地进一步纯化 ，d）从脑毛细血管内皮细胞和减数组织制备减数cDNA文库，e）使用一个或多个差异杂交进行cDNA减法，f）来自减法cDNA文库的克隆通过差异鉴定 相对于它们各自的表达进行杂交，g）从减数cDNA制备用于BBB特异性克隆的完整cDNA序列 文库，和h）比较研究的克隆的表达模式在新鲜和培养的脑毛细血管内皮细胞之间，并且鉴定出BBB特异性蛋白或其片段的存在以及鉴定的蛋白质及其片段 方法。