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公开(公告)号:US20210348174A1
公开(公告)日:2021-11-11
申请号:US17371449
申请日:2021-07-09
发明人: Min-Ju Chang , Natalia Oganesyan , Andrew Lees
摘要: The present invention is directed to the cells, compositions and methods for the production of recombinant protein, wherein an f-met group on the 5′-terminus is enzymatically removed. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.
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公开(公告)号:US11060123B2
公开(公告)日:2021-07-13
申请号:US16819775
申请日:2020-03-16
发明人: Natalia Oganesyan , Andrew Lees , Min-Ju Chang
IPC分类号: A61K39/00 , A61K39/385 , A61K39/02 , A61K39/40 , A61K48/00 , A01N63/00 , C12P21/06 , C12P21/02 , C07K14/34 , C12N15/70 , C07K1/16 , C12N1/20 , C12R1/19
摘要: The present invention is directed to the cells, compositions and methods for the production of recombinant protein, wherein an f-met group on the 5′-terminus is enzymatically removed. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.
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