公开(公告)号：US20240059755A1

公开(公告)日：2024-02-22

申请号：US18078556

申请日：2022-12-09

Applicant: Genentech, Inc.

Inventor： Whitney BINDER ,  Craig D. BLANCHETTE ,  Martine A. DARWISH ,  Rami HANNOUSH ,  Xinxin GAO

IPC: C07K14/775 ,  C07K16/28

CPC classification number: C07K14/775 ,  C07K16/2875 ,  C07K2317/55 ,  C07K2319/30 ,  C07K2319/31

Abstract: The present invention generally relates to nanolipoprotein particle conjugates comprising at least one polypeptide and nanolipoprotein particle conjugates comprising at least one short peptide between 20-60 amino acids in length. Also provided are related compositions and systems, and methods of preparing and using same. In particular, nanolipoprotein particle (NLP) conjugates with antigen-binding fragments (Fabs) are provided, where the NLP comprises a bilayer of membrane-forming lipids encircled by scaffold proteins, and the Fabs are conjugated to one or both bilayer surfaces. In some embodiments, the (NLP) conjugates further comprise a short peptide 20-60 amino acids in length. The conjugates, and related compositions and systems, generally show good stability and manufacturability, retain antigen-binding activity, and can enhance antigen-binding potency, providing versatile platforms for delivery of Fab and Fab-based therapeutics and diagnostics, as well as providing stable platforms for delivering other therapeutic and/or diagnostic agents.