公开(公告)号：US20220325277A1

公开(公告)日：2022-10-13

申请号：US17717119

申请日：2022-04-10

Applicant: Inscripta, Inc.

Inventor： Burak Dura ,  Christine Bendzinkski ,  Kara Juneau

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6855

Abstract: Provided are methods and compositions for detecting genome editing events at the single cell level. The methods and compositions described herein utilize sequence-based methods with combinatorial barcoding to track the identity of single cells over single or multiple genome editing events.

公开(公告)号：US20210246441A1

公开(公告)日：2021-08-12

申请号：US17167018

申请日：2021-02-03

Applicant: Inscripta, Inc.

Inventor： Paul Hardenbol ,  Kara Juneau ,  Michael Graige

IPC: C12N15/10 ,  C12Q1/6876

Abstract: The present disclosure relates to methods and compositions to enable use of editing cassettes for nucleic-acid guided editing and then repurposing the cassettes to increase targeted sequencing efficiency and reduce sequencing costs of the edited genomes.

公开(公告)号：US20210054451A1

公开(公告)日：2021-02-25

申请号：US16995891

申请日：2020-08-18

Applicant: Inscripta, Inc.

Inventor： Kara Juneau

IPC: C12Q1/6874 ,  C40B20/00

Abstract: Provided are methods and compositions for preparing nucleic acid fragments for sequencing by synthesis on a flow cell. The methods and compositions described herein introduce nucleotide diversity into a sample preparation that would otherwise lack nucleotide diversity due to homogeneity of the sequencing target.