公开(公告)号：US20190264216A1

公开(公告)日：2019-08-29

申请号：US16409586

申请日：2019-05-10

申请人： Intact Genomics, Inc.

发明人： Chengcang Charles Wu

IPC分类号： C12N15/80 ,  C12N15/69 ,  C12N15/52 ,  C12N15/10

摘要： Fungal artificial chromosome (FAC) vectors are disclosed. A vector can be replicated in a bacterial or a fungal host, and can comprise an insert of heterologous DNA up to about 500 kilobases. A vector can be used for cloning and expressing a secondary metabolite (SM) gene cluster. An insert sequence can be modified by homologous recombination. A vector can be a plasmid comprising bacterial and fungal origins of replication, as well as bacterial and fungal selection marker genes. Also disclosed are vectors that can be integrated into a fungal genome, and dual function vectors which can be replicated in a bacterial or a fungal host and can also be integrated into a fungal genome. Also disclosed are methods of generating plasmid libraries including vectors comprising intact SM gene clusters.

公开(公告)号：US10337019B2

公开(公告)日：2019-07-02

申请号：US15143493

申请日：2016-04-29

申请人： Intact Genomics, Inc

发明人： Chengcang Charles Wu

IPC分类号： C12N15/80 ,  C12N15/52 ,  C12N15/69 ,  C12N15/10

摘要： Fungal artificial chromosome (FAC) vectors are disclosed. A vector can be replicated in a bacterial or a fungal host, and can comprise an insert of heterologous DNA up to about 500 kilobases. A vector can be used for cloning and expressing a secondary metabolite (SM) gene cluster. An insert sequence can be modified by homologous recombination. A vector can be a plasmid comprising bacterial and fungal origins of replication, as well as bacterial and fungal selection marker genes. Also disclosed are vectors that can be integrated into a fungal genome, and dual function vectors which can be replicated in a bacterial or a fungal host and can also be integrated into a fungal genome. Also disclosed are methods of generating plasmid libraries including vectors comprising intact SM gene clusters.