公开(公告)号：US20070004656A1

公开(公告)日：2007-01-04

申请号：US10538840

申请日：2003-12-11

申请人： Joachim Schmitt ,  Gunter Boehm ,  Bernd Stahl ,  Roland Schauer ,  Evelin Tiralongo ,  Silke Schrader

发明人： Joachim Schmitt ,  Gunter Boehm ,  Bernd Stahl ,  Roland Schauer ,  Evelin Tiralongo ,  Silke Schrader

IPC分类号： A61K48/00 ,  A61K39/00 ,  C12Q1/68 ,  G01N33/569 ,  C07H21/04 ,  C12N9/24 ,  C12N1/10 ,  A61K39/002

CPC分类号： C12N9/1081

摘要： The invention relates to novel enzymes, which transfer sialic acid from a donor molecule onto an acceptor molecule (trans-sialidases). The enzymes are isolated from the protozoan Trypanosoma congolense. The invention also relates to functional equivalents of said enzymes, to the nucleic acid sequences and amino acid sequences that code for the enzymes and their functional equivalents, to expression constructs and vectors that contain said sequences, to recombinant microorganisms that carry the inventive coding nucleic-acid sequences, to a method for the recombinant production of the inventive enzymes, to a method for isolating said enzyme from Trypanosoma congolense, to a method for the enzymatic sialization of acceptor molecules using the inventive enzymes, to effectors of the inventive trans-sialidases, to the use of the nucleic acid sequences, amino acid sequences, enzymes, effectors or sialization products for producing vaccines, medicaments, foodstuffs or food additives, in addition to the latter products obtained by the inventive method.

摘要翻译： 本发明涉及将唾液酸从供体分子转移到受体分子（反唾液酸酶）上的新型酶。 这些酶是从原生动物锥虫中分离的。 本发明还涉及所述酶的功能等同物，编码酶及其功能等同物的核酸序列和氨基酸序列，含有所述序列的表达构建体和载体，携带本发明编码核酸的重组微生物， 酸序列，重组生产本发明酶的方法，用于将所述酶与锥虫属分离的方法，使用本发明酶将受体分子酶促唾液酸化的方法用于本发明的反唾液酸酶的实施者， 使用除了通过本发明方法获得的后一种产品之外的核酸序列，氨基酸序列，酶，效应物或用于生产疫苗，药物，食品或食品添加剂的唾液化产品。

公开(公告)号：US07655784B2

公开(公告)日：2010-02-02

申请号：US10538840

申请日：2003-12-11

申请人： Joachim Schmitt ,  Gunter Boehm ,  Bernd Stahl ,  Roland Schauer ,  Evelin Tiralongo ,  Silke Schrader

发明人： Joachim Schmitt ,  Gunter Boehm ,  Bernd Stahl ,  Roland Schauer ,  Evelin Tiralongo ,  Silke Schrader

IPC分类号： C07H21/02 ,  C07H21/04 ,  A61K39/00

CPC分类号： C12N9/1081

摘要： The invention relates to novel enzymes, which transfer sialic acid from a donor molecule onto an acceptor molecule (trans-sialidases). The enzymes are isolated from the protozoan Trypanosoma congolense. The invention also relates to functional equivalents of said enzymes, to the nucleic acid sequences and amino acid sequences that code for the enzymes and their functional equivalents, to expression constructs and vectors that contain said sequences, to recombinant microorganisms that carry the inventive coding nucleic-acid sequences, to a method for the recombinant production of the inventive enzymes, to a method for isolating said enzyme from Trypanosoma congolense, to a method for the enzymatic sialization of acceptor molecules using the inventive enzymes, to effectors of the inventive trans-sialidases, to the use of the nucleic acid sequences, amino acid sequences, enzymes, effectors or sialization products for producing vaccines, medicaments, foodstuffs or food additives, in addition to the latter products obtained by the inventive method.

摘要翻译： 本发明涉及将唾液酸从供体分子转移到受体分子（反唾液酸酶）上的新型酶。 这些酶是从原生动物锥虫中分离的。 本发明还涉及所述酶的功能等同物，编码酶及其功能等同物的核酸序列和氨基酸序列，含有所述序列的表达构建体和载体，携带本发明编码核酸的重组微生物， 酸序列，重组生产本发明酶的方法，用于将所述酶与锥虫属分离的方法，使用本发明酶将受体分子酶促唾液酸化的方法用于本发明的反唾液酸酶的实施者， 使用除了通过本发明方法获得的后一种产品之外的核酸序列，氨基酸序列，酶，效应物或用于生产疫苗，药物，食品或食品添加剂的唾液化产品。