公开(公告)号：US20230112730A1

公开(公告)日：2023-04-13

申请号：US17887727

申请日：2022-08-15

Applicant: Life Technologies Corporation

Inventor： David Chi ,  Aren Ewing ,  Na Li ,  Zunping Luo ,  Amir Marcovitz

IPC: C12Q1/6886

Abstract: Provided are methods and compositions for preparing a library of target nucleic acid sequences that are useful for assessing gene mutations for oncology biomarker profiling of samples. In particular, a target-specific primer panel is provided that allows for selective amplification of oncology biomarker target sequences in a sample. In one aspect, the invention relates to target-specific primers useful for selective amplification of one or more target sequences associated with oncology biomarkers from two or more sample types. In some aspects, amplified target sequences obtained using the disclosed methods, and compositions can be used in various processes including nucleic acid sequencing and used to detect the presence of genetic variants of one or more targeted sequences associated with oncology.

公开(公告)号：US11447832B2

公开(公告)日：2022-09-20

申请号：US16948046

申请日：2020-08-28

Applicant: Life Technologies Corporation

Inventor： David Chi ,  Aren Ewing ,  Na Li ,  Zunping Luo ,  Amir Marcovitz

IPC: C12Q1/6886

Abstract: Provided are methods and compositions for preparing a library of target nucleic acid sequences that are useful for assessing gene mutations for oncology biomarker profiling of samples. In particular, a target-specific primer panel is provided that allows for selective amplification of oncology biomarker target sequences in a sample. In one aspect, the invention relates to target-specific primers useful for selective amplification of one or more target sequences associated with oncology biomarkers from two or more sample types. In some aspects, amplified target sequences obtained using the disclosed methods, and compositions can be used in various processes including nucleic acid sequencing and used to detect the presence of genetic variants of one or more targeted sequences associated with oncology.