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    Quantitative, high-throughput screening method for protein stability
    4.
    发明申请
    Quantitative, high-throughput screening method for protein stability 失效
    蛋白质稳定性的定量，高通量筛选方法

    公开(公告)号：US20070099305A1

    公开(公告)日：2007-05-03

    申请号：US11634019

    申请日：2006-12-05

    申请人： Terence Oas ,  Sina Ghaemmaghami ,  Kendall Powell ,  Michael Fitzgerald

    发明人： Terence Oas ,  Sina Ghaemmaghami ,  Kendall Powell ,  Michael Fitzgerald

    IPC分类号： G01N33/558

    CPC分类号： G01N33/6848 ,  G01N33/6803 ,  G01N33/6851 ,  G01N33/726 ,  G01N2333/805 ,  G01N2458/15 ,  G01N2800/00 ,  Y10S436/811 ,  Y10T436/24

    摘要： In proteomic research, it is often necessary to screen a large number of polypeptides for the presence of stable structure. Described herein are methods (referred to as MALDI MS-HX and SUPREX) for measuring the stability of proteins in a rapid, high-throughput fashion. The method employs hydrogen exchange to estimate the stability of quantities of unpurified protein extracts, using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. A method of quantitatively determining the stability of a test protein under native conditions is disclosed. The method includes the steps (a) providing a test protein; (b) contacting the protein with an exchange buffer comprising a denaturant and deuterium, the exchange buffer having a denaturant concentration; (c) contacting the test protein with a mass spectrometry matrix medium; (d) determining a change in mass of the test protein by mass spectrometry; (e) varying the denaturant concentration of the exchange buffer; (f) repeating steps (a)-(e) a desired number of times; and (g) quantitatively determining protein stability based on the change in mass of the test protein as a function of denaturant concentration, whereby the stability of a test protein under native conditions is quantitatively determined.

    摘要翻译： 在蛋白质组学研究中，通常有必要筛选大量的多肽以保持稳定的结构。 本文描述了用于以快速，高通量方式测量蛋白质的稳定性的方法（称为MALDI MS-HX和SUPREX）。 该方法使用氢交换来估计未纯化蛋白质提取物的量的稳定性，使用基质辅助激光解吸/电离（MALDI）质谱法。 公开了一种在天然条件下定量测定测试蛋白质的稳定性的方法。 该方法包括步骤（a）提供测试蛋白; （b）使所述蛋白质与包含变性剂和氘的交换缓冲液接触，所述交换缓冲液具有变性剂浓度; （c）使测试蛋白与质谱基质介质接触; （d）通过质谱确定测试蛋白的质量变化; （e）改变交换缓冲液的变性剂浓度; （f）重复步骤（a） - （e）期望次数; 和（g）基于测试蛋白的质量变化作为变性剂浓度的函数定量测定蛋白质稳定性，从而定量测定天然条件下的测试蛋白质的稳定性。

    Communications system and method
    6.
    发明授权
    Communications system and method 有权
    通信系统和方法

    公开(公告)号：US08437750B2

    公开(公告)日：2013-05-07

    申请号：US11638452

    申请日：2006-12-14

    申请人： Michael Fitzgerald ,  Guy Waugh ,  Richard Lord ,  Michael Godley ,  Christopher Young ,  Liam Kenny ,  Ian Walter ,  Tadhg Crotty

    发明人： Michael Fitzgerald ,  Guy Waugh ,  Richard Lord ,  Michael Godley ,  Christopher Young ,  Liam Kenny ,  Ian Walter ,  Tadhg Crotty

    IPC分类号： H04Q7/20

    CPC分类号： H04W76/11 ,  H04Q2213/13098 ,  H04Q2213/13103 ,  H04Q2213/13109 ,  H04Q2213/13141 ,  H04Q2213/13217 ,  H04W8/02 ,  H04W92/12

    摘要： A mobile network comprises remote base transceiver station (BTS) nodes (3, 4) linked with a central base station controller (BSC) node (9). The latter is in turn linked with a mobile switching center (MSC, 10), a visitor location register (VLR, 11), and a home location register (HLR, 8). Mobile stations (2, MS-A, MS-B, and MS-C) are located in the cells of the BTSs (3, 4). The remote nodes (3, 4, 3(a), 4(a)) are connected to the central node BSC (9) over a remote backhaul satellite connection. Calls are routed locally while the central nodes continue to manage calls and services from central feature-rich and typically more reliable switches of a core network. This means that calls set up between subscribers on the same remote node get connected within the remote node but call supervision is still carried out by the central nodes. The features and services of the existing core network are preserved. For example, if the calling subscriber is a pre-paid GSM subscriber and their account runs out during the call a release signal will still be sent from the central node to the remote even though the voice traffic is being handled by the remote node.

    摘要翻译： 移动网络包括与中央基站控制器（BSC）节点（9）链接的远程基站收发台（BTS）节点（3,4）。 后者又与移动交换中心（MSC，10），访问者位置寄存器（VLR，11）和归属位置寄存器（HLR，8）链接。 移动台（2，MS-A，MS-B和MS-C）位于BTS（3,4）的小区中。 远程节点（3,4，3（a），4（a））通过远程回程卫星连接连接到中央节点BSC（9）。 呼叫本地路由，而中央节点继续管理来自核心网络的中心功能丰富且通常更可靠的交换机的呼叫和服务。 这意味着在同一个远程节点的用户之间建立的呼叫在远程节点内连接，但呼叫监控仍由中央节点进行。 现有核心网络的功能和服务得以保留。 例如，如果主叫用户是预付费GSM用户，并且在呼叫期间他们的账户用尽，即使语音流量正在被远程节点处理，释放信号仍将从中央节点发送到远端。

    Mount for input/output connector panel
    7.
    发明授权
    Mount for input/output connector panel 失效
    安装用于输入/输出连接器面板

    公开(公告)号：US06942501B2

    公开(公告)日：2005-09-13

    申请号：US10373672

    申请日：2003-02-24

    申请人： John Kissinger ,  David Pincek ,  Michael Fitzgerald ,  Quan Do

    发明人： John Kissinger ,  David Pincek ,  Michael Fitzgerald ,  Quan Do

    IPC分类号： H02G3/12 ,  H02G3/14 ,  H01R13/46

    CPC分类号： H02G3/123 ,  H02G3/14

    摘要： A concealable mount for connectors, such as input/output jacks or ports, is presented. The mount comprises a base with and opening for mounting to a support structure. A front panel that is movably connected to the base, is moveable between a first closed position in which the front panel extends along the front face of the base over the opening therein, and a second open position in which at least a portion of the front panel is positioned outwardly from the base. A support extends rearwardly from the front panel and is adapted to mount connectors associated with the panel. A piston arrangement is used to bias the front panel to its open position, and a latch mechanism selectively maintains the front panel in its closed position. In its closed position, the front panel conceals the connectors, and when in its open position, the front panel exposes the support and the connectors.

    摘要翻译： 提供了用于连接器的可隐藏安装件，例如输入/输出插孔或端口。 该安装件包括具有并用于安装到支撑结构的开口的基座。 可移动地连接到基座的前面板可在第一关闭位置和第二打开位置之间移动，第一关闭位置中前面板沿着基座的前表面延伸穿过其中的开口;第二打开位置，其中至少一部分前部 面板从基座向外定位。 支撑件从前面板向后延伸，并且适于安装与面板相关联的连接器。 活塞装置用于将前面板偏压到其打开位置，并且闩锁机构选择性地将前面板保持在其关闭位置。 在其关闭位置，前面板隐藏连接器，当处于其打开位置时，前面板暴露了支撑件和连接器。

    Form based biometric data collection and authentication
    8.
    发明申请
    Form based biometric data collection and authentication 有权

    公开(公告)号：US20210136062A1

    公开(公告)日：2021-05-06

    申请号：US16673642

    申请日：2019-11-04

    申请人： Michael Fitzgerald

    发明人： Michael Fitzgerald

    IPC分类号： H04L29/06

    摘要： A biometric data collection and authentication system which validates the user of a dynamically generated and customizable internet form. The system may be used to generate biometric data from fingerprints, retinal scans, and voice-prints. It utilizes a client side biometric collection tool to allow verification against an existing database, or assign a unique identifier for future verification. It also has the ability to parse biometric information on to user provided or created documents.

    Stretchable Sleeve and Wrap for Protecting and Securing Catheter Dressings and Tubes on a Patient
    10.
    发明申请
    Stretchable Sleeve and Wrap for Protecting and Securing Catheter Dressings and Tubes on a Patient 审中-公开
    用于保护和固定患者的导管敷料和管的可拉伸套和包裹

    公开(公告)号：US20130012883A1

    公开(公告)日：2013-01-10

    申请号：US13545937

    申请日：2012-07-10

    申请人： Michael Fitzgerald ,  Kezia Fitzgerald

    发明人： Michael Fitzgerald ,  Kezia Fitzgerald

    IPC分类号： A61M25/02 ,  A61M5/32

    CPC分类号： A61M25/02 ,  A61F13/10 ,  A61F13/143 ,  A61M5/1418 ,  A61M39/08 ,  A61M2005/1586 ,  A61M2025/0206 ,  A61M2025/0213

    摘要： An easily removable, non-irritating stretchable sleeve or chest wrap for protecting and securing a PICC line or chest catheter tubing and cap extending externally from the insertion point. The sleeve or chest wrap is easily attached to a patient's arm or body, securing the small length of the external PICC line or the central venous catheter, protecting the tubing and cap from external contaminants, and allowing easy access to the PICC line or the central venous catheter for administration of drugs or nutrients, and access for blood draws. This sleeve allows the patient freedom of movement and is comfortable enough to prevent skin irritation over long-term usage.

    摘要翻译： 易于拆卸，无刺激的可拉伸套管或胸包，用于保护和固定PICC线或胸部导管管和从插入点向外延伸的帽。 袖子或胸部包裹物易于连接到患者的手臂或身体，固定外部PICC线或中心静脉导管的较小长度，保护管道和帽免受外部污染物的影响，并允许轻松访问PICC线或中央 用于药物或营养物质的静脉导管，以及抽血通路。 这种袖子允许患者运动自由，并且足够舒适以防止长期使用时皮肤刺激。