公开(公告)号：US20080044903A1

公开(公告)日：2008-02-21

申请号：US11829313

申请日：2007-07-27

申请人： Mitsuhiro UENO ,  Hirofumi Yoshioka ,  Haruko Konishi ,  Kimikazu Hashino ,  Mio Morishita ,  Hideto Chono ,  Tsuyoshi Miyamura ,  Mutsumi Sano ,  Kiyozo Asada ,  Kei Fujinaga ,  Ikunoshin Kato

发明人： Mitsuhiro UENO ,  Hirofumi Yoshioka ,  Haruko Konishi ,  Kimikazu Hashino ,  Mio Morishita ,  Hideto Chono ,  Tsuyoshi Miyamura ,  Mutsumi Sano ,  Kiyozo Asada ,  Kei Fujinaga ,  Ikunoshin Kato

IPC分类号： C12N5/02

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2810/10 ,  C12N2810/80 ,  C12N2810/851 ,  C12N2810/859

摘要： Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferring receptor, or introducing a new functional group into the functional substance.

摘要翻译： 通过使用逆转录病毒将基因转移到靶细胞中的改进方法，其中基因转移效率得到改善，并且通过将逆转录病毒结合到固定在载体上并具有结合逆转录病毒活性的功能物质来有效转化靶细胞 随后洗涤; 使用能够特异性识别细胞的抗体，层粘连蛋白或富甘糖型糖链作为具有与靶细胞结合的活性的物质; 预处理靶细胞以使转移受体失活，或将新的官能团引入功能物质。

公开(公告)号：US06787359B1

公开(公告)日：2004-09-07

申请号：US09743354

申请日：2001-01-09

申请人： Mitsuhiro Ueno ,  Hirofumi Yoshioka ,  Haruko Konishi ,  Kimikazu Hashino ,  Mio Morishita ,  Hideto Chono ,  Tsuyoshi Miyamura ,  Mutsumi Sano ,  Kiyozo Asada ,  Kei Fujinaga ,  Ikunoshin Kato

发明人： Mitsuhiro Ueno ,  Hirofumi Yoshioka ,  Haruko Konishi ,  Kimikazu Hashino ,  Mio Morishita ,  Hideto Chono ,  Tsuyoshi Miyamura ,  Mutsumi Sano ,  Kiyozo Asada ,  Kei Fujinaga ,  Ikunoshin Kato

IPC分类号： C12N1563

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2810/10 ,  C12N2810/80 ,  C12N2810/851 ,  C12N2810/859

摘要： Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferrin receptor, or introducing a new functional group into the functional substance.

摘要翻译： 通过使用逆转录病毒将基因转移到靶细胞中的改进方法，其中基因转移效率得到改善，并且通过将逆转录病毒结合到固定在载体上并具有结合逆转录病毒活性的功能物质来有效转化靶细胞 随后洗涤; 使用能够特异性识别细胞的抗体，层粘连蛋白或富甘糖型糖链作为具有与靶细胞结合的活性的物质; 预处理靶细胞以使转铁蛋白受体失活，或将新的官能团引入功能物质。