公开(公告)号：US20120196385A1

公开(公告)日：2012-08-02

申请号：US13353508

申请日：2012-01-19

申请人： Noriaki YAMAMOTO ,  Takatoshi KAYA

发明人： Noriaki YAMAMOTO ,  Takatoshi KAYA

IPC分类号： G01N33/548

CPC分类号： G01N33/553 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/54373 ,  G01N2610/00

摘要： An assay method with use of a sensor chip which includes a metal member, a self-assembled monolayer (SAM), and ligands on a support, and is configured to be used for a fluorescence measuring apparatus with utilization of a surface plasmon-field enhanced Fluorescence Spectrometry, including the steps of: forming a hydrophilic high molecule layer on the self-assembled monolayer in the sensor chip; immobilizing the ligands at least one of in the hydrophilic high molecule layer and on the surface of the hydrophilic high molecule layer; and bringing a moisturizer in contact with the hydrophilic high molecule layer.

摘要翻译： 一种使用包括金属部件，自组装单层（SAM）和载体上的配体的传感器芯片的测定方法，并且被配置为用于使用表面等离子体场增强的荧光测量装置 荧光光谱法，包括以下步骤：在传感器芯片上的自组装单层上形成亲水性高分子层; 将配体固定在亲水性高分子层和亲水性高分子层表面上的至少一种; 并使保湿剂与亲水性高分子层接触。