公开(公告)号：US20210207087A1

公开(公告)日：2021-07-08

申请号：US17057021

申请日：2019-05-22

Applicant: Osaka University

Inventor： Kohji Nishida ,  Ryuhei Hayashi ,  Kimihito Nomi

IPC: C12N5/079 ,  G01N33/50

Abstract: Provided is a method for inducing differentiation into conjunctival epithelial cells, conjunctival goblet cells, and conjunctival epithelial stem and progenitor cells, the method comprising culturing colonies of pluripotent stem cells in a medium containing an epidermal growth factor (EGF) signaling activator to induce differentiation of the pluripotent stem cells. The present invention enables differentiation of pluripotent stem cells including iPS cells into conjunctival epithelial cells, conjunctival goblet cells, and conjunctival epithelial stem and progenitor cells and therefore is very useful for, for example, basic research on conjunctival epithelial cells and conjunctival goblet cells, regenerative therapy for intractable ocular surface diseases, and research related to the diseases.