公开(公告)号：US20220240499A1

公开(公告)日：2022-08-04

申请号：US17607695

申请日：2020-04-24

Applicant: Regents of the University of Minnesota

Inventor： Rui Li ,  Allison Hubel

IPC: A01N1/02 ,  A61K47/26 ,  C07K14/76

Abstract: A cryopreservative composition includes a sugar component with a total concentration of sugar components in the composition of 300 mM or less; a sugar alcohol component, with a total concentration of sugar alcohol components in the composition of 2 M or less; and at least one of a polymer component and albumin, with the proviso that the composition includes less than a cryopreservative amount of dimethyl sulfoxide (DMSO). A method of cryopreserving a cell includes adding a cell to the cryopreservative composition; freezing the composition; storing the frozen composition; thawing the composition; removing the cell from the thawed composition; and culturing the cell under conditions effective for the cell to remain viable. The freezing may include cooling at a rate of 0.1° C./min to 5° C./min. The method may be performed without a washing step after thawing.