公开(公告)号：US20220011308A1

公开(公告)日：2022-01-13

申请号：US17309261

申请日：2019-11-15

Applicant: SPARK THERAPEUTICS, INC.

Inventor： Klaudia KURANDA ,  Xavier ANGUELA ,  Federico MINGOZZI

IPC: G01N33/569 ,  G06F17/18

Abstract: Disclosed herein are methods for analyzing for or detecting the presence of non-antibody inhibitors and/or enhancers of adeno-associated virus (AAV) vector cell transduction in a biological sample from a subject. Also disclosed herein are methods for analyzing for, or detecting the presence of, AAV binding antibodies that inhibit, reduce or decrease AAV vector cell transduction in a biological sample from a subject. The methods rely, in part, on the use of empty capsid AAV particles to absorb AAV binding antibodies, to detect enhancers or inhibitors of AAV vector cell transduction, when present, in a biological sample analyzed for AAV neutralizing antibodies (NAbs).