公开(公告)号：US20050112763A1

公开(公告)日：2005-05-26

申请号：US10923268

申请日：2004-08-20

申请人： Shivinder Grewal ,  Thomas Volpe ,  Ira Hall ,  Catherine Kidner ,  Robert Martienssen

发明人： Shivinder Grewal ,  Thomas Volpe ,  Ira Hall ,  Catherine Kidner ,  Robert Martienssen

IPC分类号： A61K48/00 ,  C12N20060101 ,  C12N15/113 ,  C12N15/63

CPC分类号： C12N15/113 ,  C12N15/63 ,  C12N2310/14

摘要： Methods of regulating the formation of heterochromatin at a target locus within the genome of a cell and methods of regulating the modification of a histone protein assumed on the DNA of a target locus within the genome of a cell are provided. In particular, the invention provides methods to regulate the structure of the genome of a cell, where the structure is regulated by modulating heterochromatin formation, heterochromatin architecture, histone methylation, and/or DNA methylation, by exposing the cell to an RNAi probe complementary to a target locus so as to activate the RNAi machinery to modify the genomic structure of the target locus and adjacent segments of the genome.

摘要翻译： 提供了调节细胞基因组内目标基因座上异染色质形成的方法和调节细胞基因组内基因组DNA上假定的组蛋白蛋白质修饰的方法。 特别地，本发明提供了调节细胞基因组结构的方法，其中结构通过调节异染色质形成，异染色质结构，组蛋白甲基化和/或DNA甲基化来调节，通过将细胞暴露于互补的RNAi探针 靶基因座，以激活RNAi机制以修饰靶基因座和基因组相邻区段的基因组结构。