公开(公告)号：US11564992B2

公开(公告)日：2023-01-31

申请号：US16748296

申请日：2020-01-21

Applicant: Takeda Pharmaceutical Company Limited

Inventor： Juergen Siekmann ,  Stefan Haider ,  Hanspeter Rottensteiner ,  Peter Turecek

IPC: A61K47/64 ,  C07K1/107 ,  A61K47/60 ,  A61K47/54 ,  A61K47/61 ,  A61K47/59 ,  A61K51/08

Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.

公开(公告)号：US20230096592A1

公开(公告)日：2023-03-30

申请号：US17904153

申请日：2021-02-24

Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED

Inventor： Jean-Baptiste Farcet ,  Juergen Siekmann

IPC: G01N24/08 ,  G01R33/46

Abstract: This invention provides, among other things, methods for determining the average number of first polymers covalently attached to protein-first polymer conjugates in a solution.