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公开(公告)号：US20220267796A1
公开(公告)日：2022-08-25
申请号：US17622044
申请日：2020-06-26
Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED
Inventor： Christian FIEDLER , Marcus SCHEINDEL , Meinhard HASSLACHER , Jadranka KOEHN
Abstract: Provided herein are methods of producing an adeno-associated virus (AAV) product and methods of purifying adeno-associated virus. AAV is loaded onto an affinity resin, wash steps are undertaken at room temperature, and AAV is eluted from the affinity resin at a lower temperature. Various buffers are disclosed for use in the wash steps and elution.
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