摘要:
Disclosed is a genomic DNA encoding a polypeptide capable of inducing the production of interferon-&ggr; by immunocompetent cells. The genomic DNA efficiently expresses the polypeptide with high biological activities of such as inducing the production of interferon-&ggr; by immunocompetent cells, enhancing killer cells' cytotoxicity and inducing killer cells' formation, when introduced into mammalian host cells. The high biological activities of the polypeptide facilitate its uses to treat and/or prevent malignant tumors, viral diseases, bacterial infectious diseases and immune diseases without serious side effects when administered to humans.
摘要:
The receptor for interleukin-18 (IL-18) is described. The invention provides recombinant receptor polypeptides, including soluble receptors and fusion proteins, which specifically bind to IL-18 and inhibit its activity. The polypeptides are thus useful as pharmaceutical agents to treat autoimmune and allergic diseases. They are also useful as affinity reagents to purify IL-18, in assays to detect the ligand, and in screening assays to identify IL-18 agonists or antagonists.
摘要:
Disclosed is a genomic DNA encoding a polypeptide capable of inducing the production of interferon-.gamma. by immunocompetent cells. The genomic DNA efficiently expresses the polypeptide with high biological activities of such as inducing the production of interferon-.gamma. by immunocompetent cells, enhancing killer cells' cytotoxicity and inducing killer cells' formation, when introduced into mammalian host cells. The high biological activities of the polypeptide facilitate its uses to treat and/or prevent malignant tumors, viral diseases, bacterial infectious diseases and immune diseases without serious side effects when administered to humans.
摘要:
Disclosed are a polypeptide (including that in soluble form) as receptor for a novel cytokine, i.e., interleukin-18, a DNA encoding the polypeptide, and the uses of the polypeptide including pharmaceutical and neutralizer to interleukin-18. Pharmaceuticals with the polypeptide is useful to treat and prevent autoimmune and allergic disease because it suppresses and regulates excessive immunoreaction.
摘要:
The present invention has objects to provide a mutant interferon-α protein having a higher anti-viral and anti-tumor activity and to provide an agent for susceptive diseases, which contains the mutant interferon-α protein as an effective ingredient; and solves the above objects by providing a mutant protein which has an amino acid sequence of human interferon-α subtype α8 represented by any one of SEQ ID NOs:1 to 3, where the arginine residue at the 145th has been replaced with leucine, isoleucine, or valine residue; the alanine residue at the 146th has been replaced with asparagine or serine residue; and the methionine residue at the 149th has been replaced with tyrosine residue; and an agent for susceptive diseases, containing the mutant protein.
摘要翻译:本发明的目的是提供具有较高抗病毒和抗肿瘤活性的突变型干扰素-α蛋白质,并提供含有突变型干扰素-α蛋白作为有效成分的感染性疾病药物; 通过提供具有SEQ ID NO:1〜3中任一项所示的人干扰素-α亚型α8的氨基酸序列的突变蛋白来解决上述目的,其中第145位的精氨酸残基被亮氨酸替代,异亮氨酸 ,或缬氨酸残基; 第146位的丙氨酸残基被天冬酰胺或丝氨酸残基取代; 第149位的甲硫氨酸残基被酪氨酸残基取代; 和含有突变蛋白的感染性疾病的药剂。
摘要:
A protein which induces the IFN-&ggr; production by immunocompetent cells and has a molecular weight of 19,000±5,000 daltons on SDS-PAGE or gel filtration method and a pI of 4.8±1.0 on chromatofocusing. The protein is isolated from mouse liver and can be purified by a monoclonal antibody specific to it. The monoclonal antibody can be also used for assaying the protein.
摘要:
DNA encoding a murine protein capable of inducing the production of interferon-.gamma. by immunocompetent cells is described. The native protein from mouse liver has a molecular weight of 19.+-.5 kDa, a pI of 4.8.+-.1.0, and the amino acid sequence shown in SEQ ID NO: 2. Methods for producing the protein in recombinant expression systems including E. coli are provided.
摘要翻译:描述了编码能够通过免疫活性细胞诱导干扰素-γ产生的鼠蛋白的DNA。 来自小鼠肝脏的天然蛋白质的分子量为19 +/- 5kDa,pI为4.8 +/- 1.0,氨基酸序列如SEQ ID NO:2所示。在重组表达系统中产生蛋白质的方法包括 提供大肠杆菌。
摘要:
Disclosed are a receptor protein which recognize a novel cytokine, i.e., interleukin-18, a monoclonal antibody specific to the protein, and uses thereof. The receptor protein is useful as pharmaceutical to treat and prevent autoimmune and allergic disease because it suppresses and regulates excessive immunoreaction. The monoclonal antibody specifically reacts with interleukin-18, exhibiting efficacy in purification, detection and inhibition of interleukin-18.
摘要:
A human IFN-γ inducing polypeptide and its nucleotide sequence was isolated, purified and characterized. Pharmaceutical compositions containing this novel IFN-γ inducing polypeptide or active fragments thereof are formulated and monoclonal antibodies are raised against this polypeptide or antigenic fragments thereof. The polypeptide can be used in a method for treating diseases susceptive to treatment with IFN-γ, and methods for enhancing the cytotoxicity of NK cells or for inducing the formation of LAK cells, such as to treat tumors and malignant pathologies.
摘要:
An agent for susceptive diseases, which contains a polypeptide that has a molecular weight of 18,500±3,000 daltons on SDS-PAGE and a pI of 4.9±1.0 on chromatofocusing, strongly induces the IFN-&ggr; production by immunocompetent cells with only a small amount, and does not cause serious side effects even when administered to human at a relatively-high dose. The agent treats and/or prevents malignant tumors, viral diseases, bacterial infectious diseases, and immune diseases including atopies.