公开(公告)号：US20200016591A1

公开(公告)日：2020-01-16

申请号：US16465686

申请日：2017-12-14

Applicant: University of Twente

Inventor： Richard Schasfoort ,  Arjan Tibbe ,  Joska Johannes Broekmaat ,  Fikri Abali ,  Leon WMM Terstappen

IPC: B01L3/00 ,  G01N33/543

Abstract: The present invention discloses a device and methods for the analysis of secreted molecules from a single cell. As described herein, the invention incorporates individual microwells with a bottom surface capable of capturing a cell and allows the release of secreted molecules to be printed onto a capture surface. The device provides accurate identification of the cell source of the printed molecules by mapping the printed molecules to the cell source. The invention further employs spectrometry, immunoassay or label free Surface Plasmon Resonance imaging for detection of the secreted molecules in combination with a microwell array, where single cells are seeded in individual microwells and the secreted molecules are captured by the capture surface in an array print while the cell remains in the microwell for additional interrogation. The present invention has applications in medical research and diagnosis where individual target cells in a fluid sample are interrogated for the secreted products.