公开(公告)号：US5112955A

公开(公告)日：1992-05-12

申请号：US681304

申请日：1991-04-08

申请人： Tze-Chein Wun

发明人： Tze-Chein Wun

IPC分类号： C07K14/81

CPC分类号： C07K14/8132

摘要： A method of purifying PAI-1 from a biological fluid source, e.g. conditioned media of Hep G2 or HT 1020 cells, containing PAI-1 is disclosed, which comprises subjecting the biological fluid to a modified urokinase affinity absorbent, e.g. anhydrourokinase ligand bound to a CNBr-activated agarose gel or urokinase mutated at amino acid position 356 from Ser to Gly and bound to the gel, and then eluting PAI-1 from said affinity absorbent. A method of stabilizing and/or activating PAI-1 is also disclosed, which comprises complexing PAI-1 with vitronectin.

摘要翻译： 从生物流体源纯化PAI-1的方法，例如， 公开了含有PAI-1的Hep G2或HT 1020细胞的条件培养基，其包括使生物流体经受修饰的尿激酶亲和吸收剂，例如， 与CNBr活化的琼脂糖凝胶结合的脱水尿激酶配体或在氨基酸位置356从Ser突变至Gly并与凝胶结合的尿激酶，然后从所述亲和吸收剂中洗脱PAI-1。 还公开了稳定和/或活化PAI-1的方法，其包括将PAI-1与玻连蛋白络合。

公开(公告)号：US5073626A

公开(公告)日：1991-12-17

申请号：US532410

申请日：1990-06-04

申请人： Tze-Chein Wun

发明人： Tze-Chein Wun

IPC分类号： C07K14/81

CPC分类号： C07K14/8132

摘要： A method of purifying PAI-1 from a biological fluid source, e.g. conditioned media of Hep G2 or HT 1080 cells, containing PAI-1 is disclosed, which comprises subjecting the biological fluid to a modified urokinase affinity absorbent, e.g. anhydrourokinase ligand bound to a CNBr-activated agarose gel or urokinase mutated at amino acid position 356 from Ser to Gly and bound to the gel, and then eluting PAI-1 from said affinity absorbent. A method of stabilizing and/or activating PAI-1 is also disclosed, which comprises complexing PAI-1 with vitronectin.

摘要翻译： 从生物流体源纯化PAI-1的方法，例如， 公开了含有PAI-1的Hep G2或HT 1080细胞的条件培养基，其包括使生物流体经受修饰的尿激酶亲和吸收剂，例如， 与CNBr活化的琼脂糖凝胶结合的脱水尿激酶配体或在氨基酸位置356从Ser突变至Gly并与凝胶结合的尿激酶，然后从所述亲和吸收剂中洗脱PAI-1。 还公开了稳定和/或活化PAI-1的方法，其包括将PAI-1与玻连蛋白络合。

公开(公告)号：US5028534A

公开(公告)日：1991-07-02

申请号：US097482

申请日：1987-09-15

申请人： J. Evan Sadler ,  Tze-Chein Wun

发明人： J. Evan Sadler ,  Tze-Chein Wun

IPC分类号： A61K38/00 ,  C07K14/81 ,  C12N15/15

CPC分类号： C07K14/8132 ,  A61K38/00

摘要： cDNA clones having a base sequence for human placental plasminogen activator inhibitor (PAI-2) have been developed and characterized and the amino acid sequence of the PAI-2 has been determined. The PAI-2 protein has then been expressed in prokaryotic and eukaryotic cells.

摘要翻译： 已经开发出了具有人胎盘纤溶酶原激活物抑制剂（PAI-2）碱基序列的cDNA克隆并鉴定了PAI-2的氨基酸序列。 然后在原核和真核细胞中表达PAI-2蛋白。