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30 条记录 (耗时 0.033 秒)

    Method of activating denatured protein
    13.
    发明授权
    Method of activating denatured protein 有权
    激活变性蛋白的方法

    公开(公告)号：US06342585B1

    公开(公告)日：2002-01-29

    申请号：US09202206

    申请日：1999-02-01

    申请人： Adelbert Grossmann

    发明人： Adelbert Grossmann

    IPC分类号： C07K100

    CPC分类号： C07K1/1133 ,  C07K14/48 ,  C07K14/745 ,  C12N9/50

    摘要： The invention relates to a method for forming a mixed disulfide of recombinant proteins, by solubilizing with a denaturing agent in the presence of a disulphide component, and then adding a disulphide component plus a denaturing agent, at a concentration sufficient to form a mixed disulfide.

    摘要翻译： 本发明涉及通过在二硫化物组分存在下与变性剂溶解，然后以足以形成混合二硫化物的浓度加入二硫化物组分加变性剂来形成重组蛋白质的混合二硫化物的方法。

    Recombinant core-streptavidin
    14.
    发明授权
    Recombinant core-streptavidin 失效
    重组核心 - 链霉抗生物素蛋白

    公开(公告)号：US5489528A

    公开(公告)日：1996-02-06

    申请号：US211833

    申请日：1994-04-28

    申请人： Erhard Kopetzki ,  Rainer Rudolph ,  Adelbert Grossmann

    发明人： Erhard Kopetzki ,  Rainer Rudolph ,  Adelbert Grossmann

    IPC分类号： C07K14/195 ,  C07K14/36 ,  C07K14/41 ,  C12N1/21 ,  C12N15/09 ,  C12N15/31 ,  C12P21/02 ,  C12R1/19 ,  G01N33/50 ,  G01N33/53 ,  C12N5/10 ,  C12N15/63

    CPC分类号： C07K14/36

    摘要： The present invention concerns a process for the isolation of recombinant core streptavidin in which host cells are transformed with a DNA coding for core streptavidin, the transformed host cells are cultured under suitable conditions, the DNA coding for core streptavidin is expressed and the recombinant core streptavidin is isolated from the host cells or the culture medium, wherein a DNA coding for core streptavidin is used which has(a) the nucleotide sequence shown in SEQ ID NO. 1 or(b) a nucleotide sequence corresponding to the nucleotide sequence (a) within the scope of the degeneracy of the genetic code.

    摘要翻译： PCT No.PCT / EP92 / 02463。 371日期1994年04月28日 102（e）日期1994年4月28日PCT提交1992年10月28日PCT公布。 公开号WO93 / 09144 日本1993年5月13日。本发明涉及分离重组核心链亲和素的方法，其中宿主细胞用编码核心链霉亲和素的DNA转化，转化的宿主细胞在合适的条件下培养，编码核心链霉抗生物素蛋白的DNA为 表达，并且从宿主细胞或培养基中分离重组核心链霉抗生物素蛋白，其中使用编码核心链亲和素的DNA，其具有（a）SEQ ID NO： 1或（b）对应于遗传密码简并范围内的核苷酸序列（a）的核苷酸序列。

    Micro flow filtration system and flow filtration method
    15.
    发明授权
    Micro flow filtration system and flow filtration method 有权

    公开(公告)号：US10213742B2

    公开(公告)日：2019-02-26

    申请号：US14386097

    申请日：2013-03-25

    申请人： Norbert Oranth ,  Nadine Losleben ,  Sascha Lutz ,  Adelbert Grossmann

    发明人： Norbert Oranth ,  Nadine Losleben ,  Sascha Lutz ,  Adelbert Grossmann

    IPC分类号： B01D61/14 ,  B01D61/18 ,  B01D61/20 ,  B01D61/22

    摘要： A micro flow filtration system comprises a fluid circuitry (3) and a first reservoir (1) outside the circuitry (3) suitable for containing a fluid. The fluid circuitry (3) comprises a tangential flow filtration module (10) capable of separating the fluid sample into a retentate stream and a permeate stream upon passage of the fluid sample into the tangential flow filtration module (10) through an inlet feed (18). The fluid circuitry (3) further comprises a second reservoir (2) integrated in the fluid circuitry (3), a pump (5) for creating and driving a fluid flow, optionally at least one pressure sensor (6, 7 or 8) for acquiring and detecting data about the fluid sample, optionally a pressure regulator (9) for regulating the flow in the fluid circuitry (3) and a plurality of conduits (22) forming the fluid circuitry (3) together with the second reservoir (2), the TFF-module (10), the pump (5), the pressure sensor (6, 7 or 8) (if present) and the pressure regulator (9) (if present). The volume of the first reservoir (1) outside the circuitry (3) is significantly larger than the volume of the second reservoir (2). The first reservoir (1) outside the fluid circuitry (3) is connected to the circuitry (3) via a connection conduit (31) such that the fluid flows unidirectionally into the circuitry (3) until the first reservoir (1) is empty so that a continuous fluid flow from the first reservoir (1) to the circuitry (3) is established.

    PURIFICATION OF NOT-GLYCOSYLATED POLYPEPTIDES
    16.
    发明申请
    PURIFICATION OF NOT-GLYCOSYLATED POLYPEPTIDES 审中-公开
    非糖苷化多糖的纯化

    公开(公告)号：US20110034672A1

    公开(公告)日：2011-02-10

    申请号：US12811397

    申请日：2009-01-15

    申请人： Roberto Falkenstein ,  Birgit Weydanz ,  Nicole Fuehrler ,  Claudia Giessel ,  Sybille Greithanner ,  Adelbert Grossmann ,  Friederike Hesse ,  Marc Pompiati ,  Andreas Schaubmar ,  Brigitte Kraemer

    发明人： Roberto Falkenstein ,  Birgit Weydanz ,  Nicole Fuehrler ,  Claudia Giessel ,  Sybille Greithanner ,  Adelbert Grossmann ,  Friederike Hesse ,  Marc Pompiati ,  Andreas Schaubmar ,  Brigitte Kraemer

    IPC分类号： C07K1/16 ,  C07K1/22 ,  C07K1/18 ,  C07K1/113 ,  C12P21/00

    CPC分类号： C07K1/1077 ,  A61K47/60 ,  C07K1/16 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C07K1/36 ,  C07K14/56 ,  C07K14/65

    摘要： The current invention reports a method for the purification of a not-glycosylated, heterologous polypeptide, which has been recombinantly produced in a prokaryotic cell, wherein the method comprises three chromatography steps of which the first chromatography step selected from i) hydrophobic charge induction chromatography, or ii) hydrophobic interaction chromatography, or iii) affinity chromatography, or iv) ion exchange chromatography, the second chromatography step is selected from i) anion exchange chromatography, or ii) cation exchange chromatography, or iii) hydroxylapatite chromatography, or iv) hydrophobic interaction chromatography, and the a third chromatography step is selected from i) hydrophobic charge induction chromatography, or ii) anion exchange chromatography, or iii) cation exchange chromatography, or iv) hydrophobic interaction chromatography, whereby the first chromatography step is an affinity chromatography in case of polypeptides capable of interacting with metal ligands, the second chromatography step is not a hydroxylapatite chromatography step in case of polypeptides with an isoelectric point below 6.0, and the third chromatography step can be performed in flow-through mode with polypeptides having a low or high isoelectric point.

    摘要翻译： 本发明报道了在原核细胞中重组产生非糖基化的异源多肽的纯化方法，其中该方法包括三个色谱步骤，其中第一色谱步骤选自i）疏水电荷诱导色谱法， 或ii）疏水相互作用层析，或iii）亲和色谱法，或iv）离子交换色谱法，第二层析步骤选自i）阴离子交换色谱法，或ii）阳离子交换色谱法，或iii）羟基磷灰石色谱法，或iv）疏水性 并且第三层析步骤选自i）疏水电荷诱导色谱法，或ii）阴离子交换色谱法，或iii）阳离子交换色谱法，或iv）疏水相互作用色谱法，其中第一层析步骤是亲和层析 能够与met相互作用的多肽的情况 第二层析步骤在等电点低于6.0的多肽的情况下不是羟基磷灰石层析步骤，并且第三层析步骤可以以具有低或高等电点的多肽的流通模式进行。