公开(公告)号：US20230121286A1

公开(公告)日：2023-04-20

申请号：US17932474

申请日：2022-09-15

Applicant: BASF ENZYMES LLC

Inventor： Danielle CUSUMANO ,  Tony NEWTON ,  Arne I. SOLBAK

IPC: C12N9/16 ,  C12C5/00 ,  C12C11/00 ,  C12G3/02 ,  C12P7/12

Abstract: The present disclosure relates to methods for using one or more polypeptides with phytase activity in grain processing, ethanol, and biofuel production.

公开(公告)号：US20210163995A1

公开(公告)日：2021-06-03

申请号：US17105100

申请日：2020-11-25

Applicant: Lallemand Hungary Liquidity Management LLC ,  BASF SE ,  BASF ENZYMES LLC.

Inventor： Aaron Argyros ,  Alexandra Panaitiu ,  Charles Rice ,  Matthew B. Richards ,  Tera Stoughtenger ,  Kenneth Barrett

IPC: C12P7/06 ,  C12N1/18 ,  C12N9/60

Abstract: The present disclosure concerns a process for fermenting a biomass with a reduced dose of a purified exogenous enzyme (which can be, for example a purified exogenous glucoamylase). The process comprises contacting a biomass (which may comprise starch) with a recombinant yeast host cell. The recombinant yeast host cell has a genetic modification for expressing a heterologous polypeptide having starch or dextrin hydrolase activity (which may be, for example, from a glucoamylase). The nucleic acid molecule encoding the heterologous polypeptide having starch or dextrin hydrolase activity comprises allowing the secretion of the heterologous polypeptide.

公开(公告)号：US20210163967A1

公开(公告)日：2021-06-03

申请号：US17105045

申请日：2020-11-25

Applicant: Lallemand Hungary Liquidity Management LLC ,  BASF SE ,  BASF ENZYMES LLC.

Inventor： Aaron Argyros ,  Alexandra Panaitiu ,  Charles Rice ,  Kenneth Barrett

IPC: C12N15/81 ,  C12N9/34 ,  C12N9/26 ,  C12P7/06

Abstract: The present disclosure concerns recombinant yeast host cell for saccharification of a biomass. The recombinant yeast host cell has a genetic modification for expressing a heterologous polypeptide having glucoamylase activity (Rasamsonia emersonii glucoamylase). In some embodiments, the heterologous polypeptide comprises the signal sequence associated with the alpha-mating 1 factor. The present disclosure also concerns a process for saccharification of a biomass using the recombinant yeast host cell as well as a process for fermenting the saccharified biomass into a fermentation product.

公开(公告)号：US10793844B2

公开(公告)日：2020-10-06

申请号：US16154205

申请日：2018-10-08

Applicant: BASF Enzymes LLC

Inventor： Kevin A. Gray ,  Nahla M. Aboushadi ,  James B. Garrett

IPC: C12N9/26 ,  A23L29/00 ,  A23L29/30 ,  A23K20/189 ,  A23K10/14 ,  C12N9/28 ,  A21D8/04 ,  C11D3/386 ,  C12C5/00 ,  C12N9/34 ,  C12N9/44

Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.

公开(公告)号：US20200094208A1

公开(公告)日：2020-03-26

申请号：US16677312

申请日：2019-11-07

Applicant: BASF Enzymes LLC

Inventor： Yun HAN ,  Michael PRATT ,  Yi WU

IPC: B01J2/16 ,  C12N9/16 ,  G06K9/00

Abstract: A liquid enzyme formulation, an enzyme granule formulation, methods for manufacturing enzyme granules using a fluid bed dryer, wherein the enzyme granules are thermostable without the need for a thermostable coating is provided. The enzyme granules are phytase granules used in the manufacturing of an animal feed, wherein the phytase granule is thermostable without the need for a thermostable coating and the phytase retains about 63% to about 134% of its activity after pelleting at 80° C.

公开(公告)号：US10550371B2

公开(公告)日：2020-02-04

申请号：US15879379

申请日：2018-01-24

Applicant: BASF Enzymes LLC

Inventor： Xuqiu Tan ,  Arne I. Solbak, Jr.

IPC: C12N9/16 ,  C12N9/24

Abstract: Polypeptides having phytase activity and polynucleotide sequences encoding the phytases are provided. The gene expresses the phytase at a level of at least 7 g/L to 40 g/L. The phytase have higher specific activity, retain activity at low pH, and retain activity at high temperature. The phytase can be used in a variety of compositions including food, feed, pharmaceuticals, and cleaning.

公开(公告)号：US10518233B2

公开(公告)日：2019-12-31

申请号：US14770766

申请日：2014-03-10

Applicant: BASF ENZYMES LLC

Inventor： Yun Han ,  Michael Pratt ,  Yi Wu

IPC: B01J2/16 ,  C12N9/16

Abstract: A liquid enzyme formulation, an enzyme granule formulation, methods for manufacturing enzyme granules using a fluid bed dryer, wherein the enzyme granules are thermostable without the need for a thermostable coating is provided. The enzyme granules are phytase granules used in the manufacturing of an animal feed, wherein the phytase granule is thermostable without the need for a thermostable coating and the phytase retains about 63% to about 134% of its activity after pelleting at 80° C.

公开(公告)号：US20190292500A1

公开(公告)日：2019-09-26

申请号：US16431356

申请日：2019-06-04

Applicant: DIREVO Industrial Biotechnology GmbH ,  BASF Enzymes, LLC

Inventor： Marco Kraemer ,  Vitaly Svetlichny ,  Klaudija Milos

IPC: C12F3/10 ,  A23K50/30 ,  C12P7/06 ,  C12P7/14 ,  C12N9/24 ,  C12N9/14 ,  A23K50/75 ,  A23K10/38 ,  C12C7/053 ,  C12N9/42

Abstract: A method of producing a fermentation product from starch containing material, the method including converting starch containing material to fermentable sugars, wherein the starch containing material is corn; fermenting the fermentable sugars with a microorganism into fermented mash; subjecting fermentation medium, during the fermentation process to an enzyme composition comprising a xylanase and a pectinase; and separating fermentation product from the fermented mash.

公开(公告)号：US20190100738A1

公开(公告)日：2019-04-04

申请号：US16062330

申请日：2016-12-16

Applicant: BASF Enzymes LLC

Inventor： Yun HAN

IPC: C12N9/26 ,  C12N9/48 ,  C12N9/50 ,  C12N9/34 ,  C12N9/42 ,  C12N9/16 ,  C12N9/24

Abstract: The present disclosure relates to liquid enzyme formulations containing one or more alpha-amylases for use in starch processing, wherein the pH of the enzyme formulation is about pH 6.0-8.0, and methods of use thereof. The present disclosure further relates to methods of making a liquid enzyme formulation containing one or more alpha-amylase having improved stability, comprising titrating the pH of the liquid enzyme formulation to a range of pH 6.0-8.0.

公开(公告)号：US10196617B2

公开(公告)日：2019-02-05

申请号：US14504849

申请日：2014-10-02

Applicant: BASF ENZYMES, LLC

Inventor： Arne Solbak ,  Brian Steer ,  Mark Dycaico ,  Katie A. Kline ,  Axel Trefzer ,  Thomas Todaro ,  Fatima El-Farrah ,  Alberto Alvarado ,  Gerhard Frey

IPC: C12N9/16 ,  C12P7/10 ,  A23L33/18 ,  A23K20/189

Abstract: This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.