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53 条记录 (耗时 0.131 秒)

    Microfluidic chemostat
    12.
    发明授权
    Microfluidic chemostat 有权
    微流控恒化器

    公开(公告)号：US07407799B2

    公开(公告)日：2008-08-05

    申请号：US11012852

    申请日：2004-12-14

    申请人： Frederick Balagadde ,  Carl L. Hansen ,  Emil Kartalov ,  Stephen R. Quake

    发明人： Frederick Balagadde ,  Carl L. Hansen ,  Emil Kartalov ,  Stephen R. Quake

    IPC分类号： C12P19/04 ,  C12M1/00

    CPC分类号： C12P19/04 ,  B01L3/5027 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0877 ,  B01L2300/10 ,  B01L2300/163 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12M23/16 ,  C12M23/34 ,  C12M39/00 ,  C12P1/00

    摘要： A chemostat is described that includes a growth chamber having a plurality of compartments. Each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber. Also, a method of preventing biofilm formation in a growth chamber of a chemostat is described. The method may include the steps of adding a lysis agent to a isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber.

    摘要翻译： 描述了一种恒温器，其包括具有多个室的生长室。 每个隔室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。 恒化器还可以包括向生长室供应生长培养基的营养供应线以及从生长室去除流体的输出端口。 另外，描述了在恒化器的生长室中防止生物膜形成的方法。 该方法可以包括以下步骤：将裂解剂加入生长室的分离部分，并将分离的部分与生长室的其余部分重新连接。

    Microfludic protein crystallography techniques
    14.
    发明授权
    Microfludic protein crystallography techniques 失效
    微氟蛋白晶体学技术

    公开(公告)号：US08709153B2

    公开(公告)日：2014-04-29

    申请号：US13280276

    申请日：2011-10-24

    申请人： Carl L. Hansen ,  Stephen R. Quake ,  James M. Berger

    发明人： Carl L. Hansen ,  Stephen R. Quake ,  James M. Berger

    IPC分类号： C30B7/14

    CPC分类号： C30B7/14 ,  F17D5/005 ,  F17D5/06 ,  Y10T137/0318 ,  Y10T137/0391

    摘要： The present invention relates to microfluidic devices and methods facilitating the growth and analysis of crystallized materials such as proteins. In accordance with one embodiment, a crystal growth architecture is separated by a permeable membrane from an adjacent well having a much larger volume. The well may be configured to contain a fluid having an identity and concentration similar to the solvent and crystallizing agent employed in crystal growth, with diffusion across the membrane stabilizing that process. Alternatively, the well may be configured to contain a fluid having an identity calculated to affect the crystallization process. In accordance with the still other embodiment, the well may be configured to contain a material such as a cryo-protectant, which is useful in protecting the crystalline material once formed.

    摘要翻译： 本发明涉及促进结晶物质如蛋白质生长和分析的微流体装置和方法。 根据一个实施例，晶体生长结构由具有大得多体积的相邻阱的可渗透膜分离。 该孔可以被配置成包含具有与晶体生长中使用的溶剂和结晶剂相似的特性和浓度的流体，并且通过膜的扩散稳定了该过程。 或者，孔可以被配置为包含具有计算以影响结晶过程的同一性的流体。 根据另一个实施方案，所述孔可以被配置为容纳诸如冷冻保护剂的材料，其可用于在形成后保护结晶材料。

    Microfluidic rotary flow reactor matrix
    16.
    发明授权
    Microfluidic rotary flow reactor matrix 有权
    微流控旋流反应器基体

    公开(公告)号：US07413712B2

    公开(公告)日：2008-08-19

    申请号：US10837025

    申请日：2004-04-30

    申请人： Jian Liu ,  Carl L. Hansen ,  Stephen R. Quake

    发明人： Jian Liu ,  Carl L. Hansen ,  Stephen R. Quake

    IPC分类号： C12Q1/68 ,  F16K11/10 ,  F15C1/04

    CPC分类号： B81B3/00 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/00833 ,  B01J2219/00891 ,  B01J2219/0095 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/68 ,  F04B43/043 ,  G01N2035/00158

    摘要： A microfluidic device comprises a matrix of rotary flow reactors. The microfluidic matrix device offers a solution to the “world-to-chip” interface problem by accomplishing two important goals simultaneously: an economy of scale in reagent consumption is achieved, while simultaneously minimizing pipetting steps. N2 independent assays can be performed with only 2N+1 pipetting steps, using a single aliquot of enzyme amortized over all reactors. The chip reduces labor relative to conventional fluid handling techniques by using an order of magnitude less pipetting steps, and reduces cost by consuming two to three orders of magnitude less reagents per reaction. A PCR format has immediate applications in medical diagnosis and gene testing. Beyond PCR, the microfluidic matrix chip provides a universal and flexible platform for biological and chemical assays requiring parsimonious use of precious reagents and highly automated processing.

    摘要翻译： 微流体装置包括旋转流反应器的基质。 微流体矩阵器件通过同时实现两个重要目标来提供“世界到芯片”接口问题的解决方案：实现了试剂消耗的规模经济，同时最大限度地减少了移液步骤。 使用在所有反应器中摊销的酶的单等分试样，可以仅使用2N + 1移液步骤进行N 2 - 2独立测定。 该芯片相对于常规流体处理技术通过使用少量的移液步骤来减少劳动，并且通过每次反应消耗两到三个数量级的试剂来降低成本。 PCR格式可以在医学诊断和基因检测中立即应用。 除了PCR之外，微流体芯片芯片为生物和化学测定提供了通用和灵活的平台，需要简约地使用贵重的试剂和高度自动化的处理。

    Microfluidic Protein Crystallography Techniques
    20.
    发明申请
    Microfluidic Protein Crystallography Techniques 失效
    微流体蛋白晶体学技术

    公开(公告)号：US20120241015A1

    公开(公告)日：2012-09-27

    申请号：US13280276

    申请日：2011-10-24

    申请人： Carl L. Hansen ,  Stephen R. Quake ,  James M. Berger

    发明人： Carl L. Hansen ,  Stephen R. Quake ,  James M. Berger

    IPC分类号： F17D1/00

    CPC分类号： C30B7/14 ,  F17D5/005 ,  F17D5/06 ,  Y10T137/0318 ,  Y10T137/0391

    摘要： The present invention relates to microfluidic devices and methods facilitating the growth and analysis of crystallized materials such as proteins. In accordance with one embodiment, a crystal growth architecture is separated by a permeable membrane from an adjacent well having a much larger volume. The well may be configured to contain a fluid having an identity and concentration similar to the solvent and crystallizing agent employed in crystal growth, with diffusion across the membrane stabilizing that process. Alternatively, the well may be configured to contain a fluid having an identity calculated to affect the crystallization process. In accordance with the still other embodiment, the well may be configured to contain a material such as a cryo-protectant, which is useful in protecting the crystalline material once formed.

    摘要翻译： 本发明涉及促进结晶物质如蛋白质生长和分析的微流体装置和方法。 根据一个实施例，晶体生长结构由具有大得多体积的相邻阱的可渗透膜分离。 该孔可以被配置成包含具有与晶体生长中使用的溶剂和结晶剂相似的特性和浓度的流体，并且通过膜的扩散稳定了该过程。 或者，孔可以被配置为包含具有计算以影响结晶过程的同一性的流体。 根据另一个实施方案，所述孔可以被配置为容纳诸如冷冻保护剂的材料，其可用于在形成后保护结晶材料。