公开(公告)号：US06528052B1

公开(公告)日：2003-03-04

申请号：US09677109

申请日：2000-09-29

申请人： R. Lane Smith ,  Dennis R. Carter ,  David J. Schurman

发明人： R. Lane Smith ,  Dennis R. Carter ,  David J. Schurman

IPC分类号： C12N500

CPC分类号： C12N5/0655 ,  A61F2/08 ,  A61F2/28 ,  A61F2/30756 ,  A61F2/3094 ,  A61F2240/001 ,  A61F2310/00365 ,  A61K35/12 ,  A61K35/32 ,  C12N2500/02 ,  C12N2521/00

摘要： A method for in vivo, ex vivo and in vitro regeneration of cartilage and collagen. In vivo, ex vivo and in vitro regeneration and de novo formation of articular cartilage and collagen by intermittently applied hydrostatic pressure. The application of external interval loading consisting of repeated periods of applied hydrostatic pressure followed and interrupted by periods of recovery. The application of the intermittent hydrostatic pressure at physiological levels 5-10 MPA for an interval of 4 hours followed by a recovery period up to about 20 hours, said pressure applied to the cartilage cells in vitro, explants of cartilage ex vivo and in vivo to cartilage that remains intact within te joint space of diarthrotic joints. The interval loading results in the selective inhibition of matrix degrading enzymes, pro-inflammatory cytokines and chemokines that attract inflammatory cells into the joint cavity and in selective decrease of gene, expression of growth factors that are inhibitory to type II collagen expression.

摘要翻译： 一种体内，体外和体外再生软骨和胶原蛋白的方法。 通过间歇施加的静水压力，体内，离体和体外再生和从头形成关节软骨和胶原。 外部间隔负荷的应用由反复施加的静水压力组成，随后由复原期间中断。 在生理水平5-10MPA下间歇4小时的间歇静水压力的应用，随后的恢复期直到约20小时，所述压力在体外施加于软骨细胞，体外离体外植体和体内至软体 在关节关节的关节间隙内保持完整的软骨。 间隔负荷导致基质降解酶，促炎细胞因子和趋化因子的选择性抑制，其吸引炎症细胞进入关节腔，并选择性降低基因，抑制II型胶原表达的生长因子的表达。