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80 条记录 (耗时 0.03 秒)

    Construction of normalized cDNA libraries from eucaryotic cells
    15.
    发明授权
    Construction of normalized cDNA libraries from eucaryotic cells 有权
    从真核细胞构建标准化cDNA文库

    公开(公告)号：US06218123B1

    公开(公告)日：2001-04-17

    申请号：US09371257

    申请日：1999-08-10

    申请人： Michael Nehls ,  Brian Zambrowicz ,  Glenn Friedrich ,  H. Earl Ruley ,  Arthur T. Sands ,  Sigrid Wattler

    发明人： Michael Nehls ,  Brian Zambrowicz ,  Glenn Friedrich ,  H. Earl Ruley ,  Arthur T. Sands ,  Sigrid Wattler

    IPC分类号： C12Q168

    CPC分类号： C12N15/1093 ,  C12N15/1072 ,  C12N15/1096

    摘要： A new technology is described that allows for the rapid and efficient construction of complex cDNA libraries from cultured eukaryotic cells. The technology exploits eukaryotic biology by using transgenic constructs that have been nonspecifically inserted into the genome to facilitate the expression of nuclear genes as fusion transcripts. The invention further allows one to specifically subclone the corresponding fusion transcripts into highly complex cDNA libraries. The libraries are easily characterized by molecular analysis techniques such as hybridization, and individual clones can be directly sequenced to generate a sequence database of the cellular portion of the fusion transcripts.

    摘要翻译： 描述了允许从培养的真核细胞快速和有效地构建复合cDNA文库的新技术。 该技术通过使用非特异性插入基因组的转基因构建体来利用真核生物学，以促进核基因作为融合转录物的表达。 本发明还允许将相应的融合转录物特异性亚克隆到高度复杂的cDNA文库中。 通过诸如杂交的分子分析技术容易地表征文库，并且可以直接测序个体克隆以产生融合转录物的细胞部分的序列数据库。

    Targeted gene discovery
    16.
    发明授权
    Targeted gene discovery 失效
    靶向基因发现

    公开(公告)号：US6139833A

    公开(公告)日：2000-10-31

    申请号：US907598

    申请日：1997-08-08

    申请人： Rob Burgess ,  Glenn Friedrich ,  Brian Zambrowicz ,  Arthur Sands

    发明人： Rob Burgess ,  Glenn Friedrich ,  Brian Zambrowicz ,  Arthur Sands

    IPC分类号： C12N15/10 ,  C12N15/85 ,  C12N15/867 ,  A01N63/00 ,  A61K39/00 ,  C12N1/20 ,  C12Q1/68

    CPC分类号： C12N15/86 ,  C12N15/102 ,  C12N15/1034 ,  C12N15/85 ,  A01K2217/075 ,  C12N2740/10043 ,  C12N2800/60 ,  C12N2840/20 ,  C12N2840/44

    摘要： The present invention describes a comprehensive system for gene discovery using retrovirus that have been engineered to exhibit increased accessibility to genomic DNA, or to mutate and identify the chromosomal target sequences of DNA binding proteins. The strategy employs the combination of retroviral integrase/DNA binding protein fusion constructs and gene-trapping methodologies. This novel technology provides the ability to establish proviral integration at any location within the genome. In addition, it allows for the generation of a collection of eukaryotic cells in which each cell contains a mutation in a target gene or sequence for a known DNA binding protein which also allow for rapid in vivo functional analysis. Sequence information obtained for genes identified using the described methods identify a collection of eukaryotic genes related by, or directly or indirectly regulated by, a given DNA binding protein.

    摘要翻译： 本发明描述了使用逆转录病毒的基因发现的综合系统，该系统已被工程化以显示对基因组DNA的增加的可及性，或突变和鉴定DNA结合蛋白的染色体靶序列。 该策略采用逆转录病毒整合酶/ DNA结合蛋白融合构建体和基因捕获方法的组合。 这种新技术提供了在基因组内的任何位置建立原病毒整合的能力。 此外，它允许产生真核细胞的集合，其中每个细胞含有目标基因中的突变或用于已知DNA结合蛋白的序列，其也允许快速的体内功能分析。 使用所述方法鉴定的基因获得的序列信息鉴定与给定DNA结合蛋白相关或直接或间接调节的真核基因的集合。

    Novel mutated mammalian cells and animals
    20.
    发明申请
    Novel mutated mammalian cells and animals 审中-公开
    新型突变的哺乳动物细胞和动物

    公开(公告)号：US20050186677A1

    公开(公告)日：2005-08-25

    申请号：US10964549

    申请日：2004-10-13

    申请人： Glenn Friedrich ,  Brian Zambrowicz ,  Arthur Sands

    发明人： Glenn Friedrich ,  Brian Zambrowicz ,  Arthur Sands

    IPC分类号： C07K14/47 ,  C12N5/06 ,  C12N5/08 ,  C12N15/86

    CPC分类号： C07K14/47

    摘要： Novel mutated mammalian cells are provided that have been characterized by identifying the sequence of the genes that have been mutated. Preferably, novel mutated cells are murine ES cells that stably incorporate retroviral gene trap constructs in the specifically identified genes. The novel mutated cells and animals are useful in functional genomic analysis, and in the discovery and development of new therapeutic and diagnostics agents and methods.

    摘要翻译： 提供了通过鉴定突变的基因的序列来表征的新型突变的哺乳动物细胞。 优选地，新的突变细胞是在特异性鉴定的基因中稳定并入逆转录病毒基因陷阱构建体的鼠ES细胞。 新型突变的细胞和动物在功能基因组分析中以及在发现和开发新的治疗和诊断试剂和方法中是有用的。