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15 条记录 (耗时 0.012 秒)

    Long-term shelf preservation of cells and multicellular specimens by vitrification
    11.
    发明申请
    Long-term shelf preservation of cells and multicellular specimens by vitrification 审中-公开
    通过玻璃化长期保存细胞和多细胞标本

    公开(公告)号：US20060240399A1

    公开(公告)日：2006-10-26

    申请号：US11471812

    申请日：2006-06-21

    申请人： Victor Bronshtein

    发明人： Victor Bronshtein

    IPC分类号： A01N1/00 ,  A01N1/02

    CPC分类号： A01N1/02 ,  A01N1/0221

    摘要： The method of preservation by vitrification, described in the present application, provides for storage of samples at higher temperatures than in conventional methods and can be applied to cells, multicellular tissues, organs and organisms. The method of the present invention includes preparing a solution of vitrification non-permeating co-solutes (amino acids, betaines, carbohydrates, or other non-permeating co-solutes that effectively decrease the chemical potential of permeating cryoprotectants in aqueous solutions), a permeating cryoprotectant and a non-permeating cryoprotectant (polyvinylpyrrolidone, polyethylene glycol, dextran, hydroxy ethyl starch, Ficoll, etc.), contacting a sample with the vitrification solution and storing the sample at a storage temperature. The method also includes the step of rehydrating the preserved sample in a rehydration solution prepared in the manner of the vitrification storage solution. The present invention is also directed to a vitrification solution and a rehydration solution as described in connection with the method.

    摘要翻译： 在本申请中描述的通过玻璃化的保存方法提供在比常规方法更高的温度下储存样品，并且可以应用于细胞，多细胞组织，器官和生物体。 本发明的方法包括制备玻璃化非渗透性共溶质（氨基酸，甜菜碱，碳水化合物或其它不渗透的共溶质，其有效降低渗透性冷​​冻保护剂在水溶液中的化学势），渗透 冷冻保护剂和不渗透的冷冻保护剂（聚乙烯吡咯烷酮，聚乙二醇，葡聚糖，羟基乙基淀粉，Ficoll等），将样品与玻璃化溶液接触并将样品储存在储存温度。 该方法还包括在以玻璃化储存溶液的方式制备的再水化溶液中将保存的样品再水化的步骤。 本发明还涉及如该方法所述的玻璃化溶液和补液溶液。

    Preservation by foam formation
    12.
    发明授权
    Preservation by foam formation 失效
    泡沫形成保存

    公开(公告)号：US5766520A

    公开(公告)日：1998-06-16

    申请号：US785473

    申请日：1997-01-17

    申请人： Victor Bronshtein

    发明人： Victor Bronshtein

    IPC分类号： F26B5/04 ,  A61K9/12 ,  A61K35/12 ,  A61K35/16 ,  A61K35/76 ,  A61K38/00 ,  A61K39/00 ,  A61K45/00 ,  A61K47/30 ,  B01D1/18 ,  B01J13/00 ,  C12N1/04 ,  C12N9/04 ,  C12N9/96 ,  B01J13/02 ,  B01J13/04 ,  B32B5/16

    CPC分类号： C12N9/0006 ,  B01J13/00 ,  C12N1/04 ,  C12N9/96 ,  Y10S516/924 ,  Y10T428/2984 ,  Y10T436/107497 ,  Y10T436/108331

    摘要： A method of preserving sensitive biological dispersions, suspensions, emulsions and solutions by forming stable foams from fluid materials to be dehydrated, as an aid both to the drying of one or more biologically active substrates in the fluid and as an aid in preparing an easily divisible dried product suitable for further commercial use. The stable foams are formed by partially removing the water to form a viscous liquid and by further subjecting the reduced liquid to vacuum, to cause it to "boil" during further drying at temperatures substantially lower than 100 degrees C. In other words, reduced pressure is applied to viscous solutions or suspensions of biologically active materials to cause the solutions or suspensions to foam during boiling, and during the foaming process further solvent removal causes the ultimate production of a stable open-cell or closed-cell foam.

    摘要翻译： 通过从待脱水的流体材料形成稳定的泡沫来辅助敏感的生物分散体，悬浮液，乳液和溶液的方法，以帮助干燥流体中的一种或多种生物活性底物，并有助于制备容易分割的 干燥产品适合进一步商业用途。 稳定的泡沫体通过部分去除水以形成粘性液体并通过进一步将还原液体进行真空的方式形成，使其在基本上低于100℃的温度进一步干燥期间“沸腾”。换句话说，减压 应用于生物活性材料的粘稠溶液或悬浮液，以使溶液或悬浮液在沸腾期间发泡，并且在发泡过程中，进一步的溶剂去除导致最终产生稳定的开孔或闭孔泡沫。

    Bulk drying and the effects of inducing bubble nucleation
    13.
    发明授权
    Bulk drying and the effects of inducing bubble nucleation 失效
    大量干燥和诱导气泡成核的作用

    公开(公告)号：US06884866B2

    公开(公告)日：2005-04-26

    申请号：US10274719

    申请日：2002-10-18

    申请人： Victor Bronshtein ,  Kevin R. Bracken ,  John G. Cambell

    发明人： Victor Bronshtein ,  Kevin R. Bracken ,  John G. Cambell

    IPC分类号： F26B5/02 ,  F26B5/04 ,  C08F6/00

    CPC分类号： F26B5/04 ,  F26B5/02

    摘要： The present invention discloses apparatus and methods of inducing bubble nucleation to overcome problems commonly associated with preservation by foam formation. Specifically, the invention relates to methods of using bubble nucleation in foam formation to preserve sensitive biological materials. Preferred methods of inducing bubble nucleation include, mixing, chamber rotation, crystals, and ultrasound.

    摘要翻译： 本发明公开了引发气泡成核的装置和方法，以克服与通过泡沫形成的保存通常相关的问题。 具体地，本发明涉及在泡沫形成中使用气泡成核以保存敏感的生物材料的方法。 引发气泡成核的优选方法包括混合，室旋转，晶体和超声波。