公开(公告)号：US20210155982A1

公开(公告)日：2021-05-27

申请号：US16951864

申请日：2020-11-18

Applicant: 10X Genomics, Inc.

Inventor： Yifeng Yin ,  Zachary Bent ,  Stephen Williams ,  Ian Fiddes ,  Jeffrey Clark Mellen ,  Jasper Staab ,  Kevin J. Wu ,  Neil Ira Weisenfeld ,  Florian Baumgartner ,  Brynn Claypoole ,  Preyas Shah ,  Narek Dshkhunyan ,  Erik Leonard Henrik Borgstrom ,  Benjamin McCreath

IPC: C12Q1/6869 ,  C12Q1/6837 ,  G06T7/194 ,  G06T7/13 ,  G06T7/00 ,  G06N5/00

Abstract: Systems and methods for spatial analysis of analytes include placing a sample on a substrate having fiducial markers and capture spots. Then, an image of the sample is acquired and sequence reads are obtained from the capture spots. Each capture probe plurality in a set of capture probe pluralities is (i) at a different capture spot, (ii) directly or indirectly associates with analytes from the sample and (iii) has a unique spatial barcode. The sequencing reads serve to detect the analytes. Sequencing reads include a spatial barcode of the corresponding capture probe plurality. Spatial barcodes localize reads to corresponding capture spots, thereby dividing them into subsets, each subset for a respective capture spot. Fiducial markers facilitate a composite representation comprising (i) the image aligned to the capture spots and (ii) a representation of each subset of sequence reads at respective positions within the image mapping to the corresponding capture spots.