公开(公告)号：US20150219625A1

公开(公告)日：2015-08-06

申请号：US14416016

申请日：2013-07-22

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Daniele Zink ,  Yao Li

IPC: G01N33/50

CPC classification number: G01N33/5014 ,  G01N33/5026 ,  G01N33/5044 ,  G01N2333/47 ,  G01N2333/4712 ,  G01N2458/00

Abstract: There is provided an in vitro assay for screening a test compound for toxicity in renal proximal tubular cells. The method comprises contacting a test compound with a test population of renal proximal tubular cells; and examining one or more cell morphology features, examining one or more cytoskeleton features, and/or determining cell numbers of the renal proximal tubular cells in the test population and comparing such cell morphology, arrangement of cytoskeletal components and/or cell count with the respective features of a control population. A change in one or more cell morphology features, a change in arrangement of one or more cytoskeleton features or a decrease in cell numbers of the test population relative to the control population is indicative that the test compound is toxic for renal proximal tubular cells.

Abstract translation: 提供了用于在肾近端肾小管细胞中筛选测试化合物毒性的体外测定。 该方法包括使测试化合物与肾近端肾小管细胞的测试群体接触; 并检查一个或多个细胞形态特征，检查一个或多个细胞骨架特征，和/或确定测试群体中肾近端肾小管细胞的细胞数，并将细胞骨架成分和/或细胞计数与相应的细胞形态 控制人口的特点。 一个或多个细胞形态特征的改变，一个或多个细胞骨架特征的排列变化或测试群体相对于对照群体的细胞数量的减少表明该测试化合物对肾近端肾小管细胞有毒性。